WB
Western blot analysis of various lysates using TriMethyl-Histone H3-K27 Rabbit pAb at 1:130000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): H3 protein. Exposure time: 1 s.IHC
Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using TriMethyl-Histone H3-K27 Rabbit pAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using TriMethyl-Histone H3-K27 Rabbit pAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using TriMethyl-Histone H3-K27 Rabbit pAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.ICC/IF
Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K27 Rabbit pAb at a dilution of 1:600 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.ChIP
Chromatin immunoprecipitation was performed with 15 μg of cross-linked chromatin from HeLa cells, using 5 μg of TriMethyl-Histone H3-K27 Rabbit pAb and Rabbit IgG isotype control. The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.| Product Name | TriMethyl-Histone H3-K27 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPICC/IFIHCWB |
| WB:1:10000-1:150000 ICC/IF:1:600-1:1200 IHC:1:2000-1:8000 ChIP:5 μg antibody for 10 μg - 15 μg of Chromatin |
|
| Species Reactivity | HumanMouseOtherRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3-4 |
|---|---|
| Gene Synonyms | H3t H3.4 H3/g H3FT H3C16 HIST3H3 |
| Gene Full Name | H3.4 histone, cluster member |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 15 kDa(Observed band size: 17 kDa) |
| Cellular Localization | Chromosome, Nucleus. |
| SwissProt ID | P68431 |
|---|
WB
Western blot analysis of various lysates using TriMethyl-Histone H3-K27 Rabbit pAb at 1:130000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): H3 protein. Exposure time: 1 s.
IHC
Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using TriMethyl-Histone H3-K27 Rabbit pAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using TriMethyl-Histone H3-K27 Rabbit pAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using TriMethyl-Histone H3-K27 Rabbit pAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ICC/IF
Immunofluorescence analysis of HeLa cells using TriMethyl-Histone H3-K27 Rabbit pAb at a dilution of 1:600 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
ChIP
Chromatin immunoprecipitation was performed with 15 μg of cross-linked chromatin from HeLa cells, using 5 μg of TriMethyl-Histone H3-K27 Rabbit pAb and Rabbit IgG isotype control. The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.| Application Notes | WB:1:10000-1:150000 ICC/IF:1:600-1:1200 IHC:1:2000-1:8000 ChIP:5 μg antibody for 10 μg - 15 μg of Chromatin |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS, pH 7.3, containing 50% glycerol. Preserved with Proclin300 or sodium azide. May contain 0.05% BSA as specified on the Certificate of Analysis. |
