WB
Western blot analysis of lysates from HeLa cells, using Acetyl-Histone H3-K4 Rabbit mAb at 1:1000 dilution. HeLa cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.WB
Western blot analysis of lysates from NIH/3T3 cells, using Acetyl-Histone H3-K4 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.DB
Dot-blot analysis of all sorts of peptides using Acetyl-Histone H3-K4 Rabbit mAb at 1:3000 dilution.ICC/IF
Immunofluorescence analysis of C6 TSA and C6 cells using Acetyl-Histone H3-K4 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.IP
Immunoprecipitation analysis of 600ug extracts of HeLa TSA cells using 5ug Acetyl-Histone H3-K4 Rabbit mAb. Western blot was performed from the immunoprecipitate using Acetyl-Histone H3-K4 Rabbit mAb at a dilition of 1:1000.ChIP
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K4 Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.| Product Name | Acetyl-Histone H3-K4 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPDBICC/IFIPWB |
| WB:1:1000-1:2000 DB:1:2000-1:5000 ICC/IF:1:50-1:200 IP:2μg-6μg antibody for 400μg-600μg extracts of whole cells ChIP: 5μg antibody for 5μg-10μg of Chromatin |
|
| Species Reactivity | HumanMouseOtherRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3-4 |
|---|---|
| Gene Synonyms | H3t H3.4 H3/g H3FT H3C16 HIST3H3 |
| Gene Full Name | H3.4 histone, cluster member |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 15 kDa(Observed band size: 17 kDa) |
| Cellular Localization | Chromosome, nucleus. |
| SwissProt ID | P68431 |
|---|
WB
Western blot analysis of lysates from HeLa cells, using Acetyl-Histone H3-K4 Rabbit mAb at 1:1000 dilution. HeLa cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
WB
Western blot analysis of lysates from NIH/3T3 cells, using Acetyl-Histone H3-K4 Rabbit mAb at 1:1000 dilution. NIH/3T3 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
DB
Dot-blot analysis of all sorts of peptides using Acetyl-Histone H3-K4 Rabbit mAb at 1:3000 dilution.
ICC/IF
Immunofluorescence analysis of C6 TSA and C6 cells using Acetyl-Histone H3-K4 Rabbit mAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
IP
Immunoprecipitation analysis of 600ug extracts of HeLa TSA cells using 5ug Acetyl-Histone H3-K4 Rabbit mAb. Western blot was performed from the immunoprecipitate using Acetyl-Histone H3-K4 Rabbit mAb at a dilition of 1:1000.
ChIP
Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H3-K4 Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.| Application Notes | WB:1:1000-1:2000 DB:1:2000-1:5000 ICC/IF:1:50-1:200 IP:2μg-6μg antibody for 400μg-600μg extracts of whole cells ChIP: 5μg antibody for 5μg-10μg of Chromatin |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
