WB
Western blot analysis of lysates from HeLa cells using Phospho-Histone H3-S10 Rabbit mAb at 1:6000 dilution incubated overnight at 4℃. HeLa cells were treated with Nocodazole (100 ng/mL) at 37℃ for 17 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1 s.IHC
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using Phospho-Histone H3-S10 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Phospho-Histone H3-S10 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using Phospho-Histone H3-S10 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.ICC/IF
Confocal imaging of NIH/3T3 cells treated with Nocodazole, NIH/3T3 cells treated with Nocodazole and λpp, and untreated NIH/3T3 cells using Phospho-Histone H3-S10 Rabbit mAb (dilution 1:1000) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.ICC/IF
Confocal imaging of HeLa cells (treated with Nocodazole) and HeLa cells (untreated) using Phospho-Histone H3-S10 Rabbit mAb (dilution 1:1000) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.IP
Immunoprecipitation of Phospho-Histone H3-S10 from 600 µg extracts of HeLa cells treated with Nocodazole (100 ng/mL,17 h) was performed using 1.5 µg of Phospho-Histone H3-S10 Rabbit mAb. Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with 1x Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Phospho-Histone H3-S10 Rabbit mAb at a dilution of 1:10000.CHIP
Chromatin immunoprecipitation was performed with 15 μg of cross-linked chromatin from HeLa cells(left) and HeLa cells were treated with Nocodazole (100 ng/mL,17 h) (right), using 1 μg of Phospho-Histone H3-S10 Rabbit mAb and Rabbit IgG isotype control. The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.| Product Name | Phospho-Histone H3-S10 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Modification | p-S10 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPICC/IFIHCIPWB |
| WB:1:1000-1:20000 IP:0.5 μg - 4 μg antibody for 400 μg - 600 μg extracts of whole cells ICC/IF:1:1000-1:5000 IHC:1:500-1:2000 ChIP:1 μg antibody for 10 μg - 15 μg of Chromatin |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3-4 |
|---|---|
| Gene Synonyms | H3t H3.4 H3/g H3FT H3C16 HIST3H3 |
| Gene Full Name | H3.4 histone, cluster member |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 15 kDa(Observed band size: 17 kDa) |
| Cellular Localization | Chromosome, Nucleus. |
| SwissProt ID | P68431 |
|---|
WB
Western blot analysis of lysates from HeLa cells using Phospho-Histone H3-S10 Rabbit mAb at 1:6000 dilution incubated overnight at 4℃. HeLa cells were treated with Nocodazole (100 ng/mL) at 37℃ for 17 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1 s.
IHC
Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using Phospho-Histone H3-S10 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Phospho-Histone H3-S10 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using Phospho-Histone H3-S10 Rabbit mAb at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.
ICC/IF
Confocal imaging of NIH/3T3 cells treated with Nocodazole, NIH/3T3 cells treated with Nocodazole and λpp, and untreated NIH/3T3 cells using Phospho-Histone H3-S10 Rabbit mAb (dilution 1:1000) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
ICC/IF
Confocal imaging of HeLa cells (treated with Nocodazole) and HeLa cells (untreated) using Phospho-Histone H3-S10 Rabbit mAb (dilution 1:1000) followed by a further incubation with Cy3-conjugated Goat anti-Rabbit IgG (H+L) (dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
IP
Immunoprecipitation of Phospho-Histone H3-S10 from 600 µg extracts of HeLa cells treated with Nocodazole (100 ng/mL,17 h) was performed using 1.5 µg of Phospho-Histone H3-S10 Rabbit mAb. Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with 1x Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using Phospho-Histone H3-S10 Rabbit mAb at a dilution of 1:10000.
CHIP
Chromatin immunoprecipitation was performed with 15 μg of cross-linked chromatin from HeLa cells(left) and HeLa cells were treated with Nocodazole (100 ng/mL,17 h) (right), using 1 μg of Phospho-Histone H3-S10 Rabbit mAb and Rabbit IgG isotype control. The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.| Application Notes | WB:1:1000-1:20000 IP:0.5 μg - 4 μg antibody for 400 μg - 600 μg extracts of whole cells ICC/IF:1:1000-1:5000 IHC:1:500-1:2000 ChIP:1 μg antibody for 10 μg - 15 μg of Chromatin |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
