WB
Western blot analysis of lysates from HeLa cells using Acetyl-Histone H4-K12 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. HeLa cells were treated with TSA (1 μM) at 37℃ for 18 hours after serum starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10 s.WB
Western blot analysis of lysates from C6 cells using Acetyl-Histone H4-K12 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. C6 cells were treated with TSA (1 μM) at 37℃ for 18 hours after serum starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10 s.IHC
Immunohistochemistry analysis of paraffin-embedded Human colon using Acetyl-Histone H4-K12 Rabbit mAb at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Acetyl-Histone H4-K12 Rabbit mAb at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.IP
Immunoprecipitation analysis of 600 μg extracts of HeLa cells using 5 μg Acetyl-Histone H4-K12 Rabbit mAb. Western blot was performed from the immunoprecipitate using Acetyl-Histone H4-K5 Rabbit mAb at a dilition of 1:10000.CHIP
Chromatin immunoprecipitation analysis of extracts of Hela cells, using Acetyl-Histone H4-K12 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.| Product Name | Acetyl-Histone H4-K12 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPIHCIPWB |
| WB:1:1000-1:3000 IHC:1:500-1:1000 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ChIP:5μg antibody for 5μg-10μg of Chromatin |
|
| Species Reactivity | HumanMouseOtherRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H4C1 |
|---|---|
| Gene Synonyms | H4FA HIST1H4A |
| Gene Full Name | H4 clustered histone 1 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 11 kDa |
| Cellular Localization | Chromosome, Nucleus. |
| SwissProt ID | P62805 |
|---|
WB
Western blot analysis of lysates from HeLa cells using Acetyl-Histone H4-K12 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. HeLa cells were treated with TSA (1 μM) at 37℃ for 18 hours after serum starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10 s.
WB
Western blot analysis of lysates from C6 cells using Acetyl-Histone H4-K12 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. C6 cells were treated with TSA (1 μM) at 37℃ for 18 hours after serum starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 10 s.
IHC
Immunohistochemistry analysis of paraffin-embedded Human colon using Acetyl-Histone H4-K12 Rabbit mAb at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Mouse spleen using Acetyl-Histone H4-K12 Rabbit mAb at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
IP
Immunoprecipitation analysis of 600 μg extracts of HeLa cells using 5 μg Acetyl-Histone H4-K12 Rabbit mAb. Western blot was performed from the immunoprecipitate using Acetyl-Histone H4-K5 Rabbit mAb at a dilition of 1:10000.
CHIP
Chromatin immunoprecipitation analysis of extracts of Hela cells, using Acetyl-Histone H4-K12 antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.| Application Notes | WB:1:1000-1:3000 IHC:1:500-1:1000 IP:0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ChIP:5μg antibody for 5μg-10μg of Chromatin |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
