WB
Western blot analysis of various lysates using DiMethyl-Histone H4-K20 Rabbit mAb at 1:2000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): H4 protein. Exposure time: 1 s.DB
Dot-blot analysis of all sorts of peptides using DiMethyl-Histone H4-K20 Rabbit mAb antibody at 1:1000 dilution.IHC
Immunohistochemistry analysis of paraffin-embedded Human cervix cancer using DiMethyl-Histone H4-K20 Rabbit mAb at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Human spleen using DiMethyl-Histone H4-K20 Rabbit mAb at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Rat lung using DiMethyl-Histone H4-K20 Rabbit mAb at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.ICC/IF
Immunofluorescence analysis of HeLa cells using DiMethyl-Histone H4-K20 Rabbit mAb at dilution of 1:600(40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | DiMethyl-Histone H4-K20 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBICC/IFIHCWB |
| WB:1:1000-1:6000 DB:1:500-1:1000 IHC:1:500-1:1000 ICC/IF:1:500-1:1000 |
|
| Species Reactivity | HumanMouseOtherRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H4C1 |
|---|---|
| Gene Synonyms | H4FA HIST1H4A |
| Gene Full Name | H4 clustered histone 1 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 11 kDa |
| Cellular Localization | Chromosome, Nucleus. |
| SwissProt ID | P62805 |
|---|
WB
Western blot analysis of various lysates using DiMethyl-Histone H4-K20 Rabbit mAb at 1:2000 dilution incubated at room temperature for 1.5 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Negative control (NC): H4 protein. Exposure time: 1 s.
DB
Dot-blot analysis of all sorts of peptides using DiMethyl-Histone H4-K20 Rabbit mAb antibody at 1:1000 dilution.
IHC
Immunohistochemistry analysis of paraffin-embedded Human cervix cancer using DiMethyl-Histone H4-K20 Rabbit mAb at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Human spleen using DiMethyl-Histone H4-K20 Rabbit mAb at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Rat lung using DiMethyl-Histone H4-K20 Rabbit mAb at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate buffer (pH 6.0) prior to IHC staining.
ICC/IF
Immunofluorescence analysis of HeLa cells using DiMethyl-Histone H4-K20 Rabbit mAb at dilution of 1:600(40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | WB:1:1000-1:6000 DB:1:500-1:1000 IHC:1:500-1:1000 ICC/IF:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
