WB
Western blot analysis of lysates from C6 cells using Symmetric DiMethyl-Histone H4-R3 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.DB
Dot-blot analysis of H3R2me0、H3R2me1、H3R2me2a、H3R2me2s、H3R8me0、H3R8me1、H3R8me2a、H3R8me2s、H3R17me0、H3R17me1、H3R17me2a、H3R17me2s、H3R26me0、H3R26me1、H3R26me2a、H3R26me2s、H4R3me0、H4R3me1、H4R3me2a、H4R3me2s peptides using Symmetric DiMethyl-Histone H4-R3 Rabbit mAb at 1:1000 dilution.ICC/IF
Confocal imaging of U-2 OS cells using Symmetric DiMethyl-Histone H4-R3 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.| Product Name | Symmetric DiMethyl-Histone H4-R3 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBICC/IFWB |
| WB:1:1000-1:2000 ICC/IF:1:50-1:200 DB:1:500-1:1000 |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H4C1 |
|---|---|
| Gene Synonyms | H4FA HIST1H4A |
| Gene Full Name | H4 clustered histone 1 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 11 kDa |
| Cellular Localization | Extracellular exosome, extracellular region, nucleoplasm, nucleus. |
| SwissProt ID | P62805 |
|---|
WB
Western blot analysis of lysates from C6 cells using Symmetric DiMethyl-Histone H4-R3 Rabbit mAb at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
DB
Dot-blot analysis of H3R2me0、H3R2me1、H3R2me2a、H3R2me2s、H3R8me0、H3R8me1、H3R8me2a、H3R8me2s、H3R17me0、H3R17me1、H3R17me2a、H3R17me2s、H3R26me0、H3R26me1、H3R26me2a、H3R26me2s、H4R3me0、H4R3me1、H4R3me2a、H4R3me2s peptides using Symmetric DiMethyl-Histone H4-R3 Rabbit mAb at 1:1000 dilution.
ICC/IF
Confocal imaging of U-2 OS cells using Symmetric DiMethyl-Histone H4-R3 Rabbit mAb (dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (dilution 1:400) followed by incubation with 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.| Application Notes | WB:1:1000-1:2000 ICC/IF:1:50-1:200 DB:1:500-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol and 0.05% BSA, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
