WB
Western blot analysis of various lysates using DiMethyl-Histone H3-K4 Rabbit pAb at 1:3000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60 s.DB
Dot-blot analysis of all sorts of peptides using DiMethyl-Histone H3-K4 Rabbit pAb at 1:10000 dilution.IHC
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using DiMethyl-Histone H3-K4 Rabbit pAb at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using DiMethyl-Histone H3-K4 Rabbit pAb at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using DiMethyl-Histone H3-K4 Rabbit pAb at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.ICC/IF
Immunofluorescence analysis of HeLa cells using DiMethyl-Histone H3-K4 Rabbit pAb at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.IP
Immunoprecipitation of DiMethyl-Histone H3-K4 from 600 µg extracts of 293F cells was performed using 5 µg of DiMethyl-Histone H3-K4 Rabbit pAb . Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with 1x reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using DiMethyl-Histone H3-K4 Rabbit pAb at a dilution of 1:5000.CHIP
Chromatin immunoprecipitation was performed with 10 μg of cross-linked chromatin from HCT 116 cells, using 5 μg of DiMethyl-Histone H3-K4 Rabbit pAb and Rabbit IgG isotype control. The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.CHIP
Chromatin immunoprecipitation was performed with 10 μg of cross-linked chromatin from using 5 μg of DiMethyl-Histone H3-K4 Rabbit pAb. DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2. The ChIP sequencing results indicate the enrichment pattern of DiMethyl-Histone H3-K4 in the representative genomic region surrounding RPL30 gene.CHIP
Chromatin immunoprecipitation was performed with 10 μg of cross-linked chromatin from HeLa using 5 μg of DiMethyl-Histone H3-K4 Rabbit pAb. DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2. The ChIP sequencing results indicate the enrichment pattern of DiMethyl-Histone H3-K4 across chromosome 8 (upper panel) and the genomic region encompassing , a representative gene enriched in DiMethyl-Histone H3-K4 (lower panel).CUT&Tag
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ HeLa with 1 μg of DiMethyl-Histone H3-K4 Rabbit pAb, followed by incubation with (). The results denote the enrichment pattern of around gene.CUT&Tag
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ HeLa with 1 μg of DiMethyl-Histone H3-K4 Rabbit pAb, followed by incubation with Goat Anti-Rabbit IgG(H+L). The CUT&Tag results denote the enrichment pattern of DiMethyl-Histone H3-K4 across chromosome 1 (upper panel) and the genomic region encompassing ATP1A1, a representative gene enriched in DiMethyl-Histone H3-K4 (lower panel).| Product Name | DiMethyl-Histone H3-K4 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPDBICC/IFIHCIPWBCUT&Tag-seq |
| WB:1:1000-1:3000 IP:0.5 μg - 5 μg antibody for 400 μg - 600 μg extracts of whole cells ICC/IF:1:200-1:1000 IHC:1:2000-1:8000 DB:1:5000-1:10000 ChIP:5 μg antibody for 5 μg - 10 μg of Chromatin ChIP-seq:5 μg antibody for 10 μg of Chromatin CUT&Tag:10⁵cells/1μg |
|
| Species Reactivity | HumanMouseOtherRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H3-4 |
|---|---|
| Gene Synonyms | H3t H3.4 H3/g H3FT H3C16 HIST3H3 |
| Gene Full Name | H3.4 histone, cluster member |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 15 kDa(Observed band size: 17 kDa) |
| Cellular Localization | Chromosome, nucleus. |
| SwissProt ID | P68431 |
|---|
WB
Western blot analysis of various lysates using DiMethyl-Histone H3-K4 Rabbit pAb at 1:3000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 60 s.
DB
Dot-blot analysis of all sorts of peptides using DiMethyl-Histone H3-K4 Rabbit pAb at 1:10000 dilution.
IHC
Immunohistochemistry analysis of paraffin-embedded Human colon tissue using DiMethyl-Histone H3-K4 Rabbit pAb at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using DiMethyl-Histone H3-K4 Rabbit pAb at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using DiMethyl-Histone H3-K4 Rabbit pAb at a dilution of 1:3000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ICC/IF
Immunofluorescence analysis of HeLa cells using DiMethyl-Histone H3-K4 Rabbit pAb at a dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
IP
Immunoprecipitation of DiMethyl-Histone H3-K4 from 600 µg extracts of 293F cells was performed using 5 µg of DiMethyl-Histone H3-K4 Rabbit pAb . Rabbit Control IgG was used to precipitate the Control IgG sample. IP samples were eluted with 1x reducing Laemmli Buffer. The Input lane represents 10% of the total input. Western blot analysis of immunoprecipitates was conducted using DiMethyl-Histone H3-K4 Rabbit pAb at a dilution of 1:5000.
CHIP
Chromatin immunoprecipitation was performed with 10 μg of cross-linked chromatin from HCT 116 cells, using 5 μg of DiMethyl-Histone H3-K4 Rabbit pAb and Rabbit IgG isotype control. The enrichment of immunoprecipitated DNA at different genomic loci was examined by quantitative PCR. The histogram compares the ratio of the immunoprecipitated DNA to the input at given loci.
CHIP
Chromatin immunoprecipitation was performed with 10 μg of cross-linked chromatin from using 5 μg of DiMethyl-Histone H3-K4 Rabbit pAb. DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2. The ChIP sequencing results indicate the enrichment pattern of DiMethyl-Histone H3-K4 in the representative genomic region surrounding RPL30 gene.
CHIP
Chromatin immunoprecipitation was performed with 10 μg of cross-linked chromatin from HeLa using 5 μg of DiMethyl-Histone H3-K4 Rabbit pAb. DNA libraries were prepared using Scale ssDNA-seq Lib Prep Kit for Illumina V2. The ChIP sequencing results indicate the enrichment pattern of DiMethyl-Histone H3-K4 across chromosome 8 (upper panel) and the genomic region encompassing , a representative gene enriched in DiMethyl-Histone H3-K4 (lower panel).
CUT&Tag
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ HeLa with 1 μg of DiMethyl-Histone H3-K4 Rabbit pAb, followed by incubation with (). The results denote the enrichment pattern of around gene.
CUT&Tag
CUT&Tag was performed using the CUT&Tag Assay Kit (pAG-Tn5) for Illumina from 10⁵ HeLa with 1 μg of DiMethyl-Histone H3-K4 Rabbit pAb, followed by incubation with Goat Anti-Rabbit IgG(H+L). The CUT&Tag results denote the enrichment pattern of DiMethyl-Histone H3-K4 across chromosome 1 (upper panel) and the genomic region encompassing ATP1A1, a representative gene enriched in DiMethyl-Histone H3-K4 (lower panel).| Application Notes | WB:1:1000-1:3000 IP:0.5 μg - 5 μg antibody for 400 μg - 600 μg extracts of whole cells ICC/IF:1:200-1:1000 IHC:1:2000-1:8000 DB:1:5000-1:10000 ChIP:5 μg antibody for 5 μg - 10 μg of Chromatin ChIP-seq:5 μg antibody for 10 μg of Chromatin CUT&Tag:10⁵cells/1μg |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | Buffer: PBS, pH 7.3, containing 50% glycerol. Preserved with Proclin300 or sodium azide. May contain 0.05% BSA as specified on the Certificate of Analysis. |
