WB
Western blot analysis of various lysates using Acetyl-Histone H2A-K5 Rabbit pAb at 1:1000 dilution. C2C12 cells and C6 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.ICC/IF
Immunofluorescence analysis of C6 cells using Acetyl-Histone H2A-K5 Rabbit pAb at dilution of 1:100.C6 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2A-K5 Rabbit pAb at dilution of 1:100.HeLa cells were treated with TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.| Product Name | Acetyl-Histone H2A-K5 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB:1:500-1:1000 ICC/IF:1:50-1:100 |
|
| Species Reactivity | HumanMouseOtherRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H2AC8 |
|---|---|
| Gene Synonyms | H2A.1 H2A.2 H2A/a H2AFA HIST1H2AE |
| Gene Full Name | H2A clustered histone 8 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2A family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 14 kDa |
| Cellular Localization | Chromosome, Nucleus. |
| SwissProt ID | P04908 |
|---|
WB
Western blot analysis of various lysates using Acetyl-Histone H2A-K5 Rabbit pAb at 1:1000 dilution. C2C12 cells and C6 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
ICC/IF
Immunofluorescence analysis of C6 cells using Acetyl-Histone H2A-K5 Rabbit pAb at dilution of 1:100.C6 cells were treated with TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2A-K5 Rabbit pAb at dilution of 1:100.HeLa cells were treated with TSA (1 uM) at 37℃ for 18 hours. Blue: DAPI for nuclear staining.| Application Notes | WB:1:500-1:1000 ICC/IF:1:50-1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
