WB
Western blot analysis of lysates from MCF7 cells, using Lactic acid -Histone H4-K5 Rabbit pAb at 1:1000 dilution. MCF7 cells were treated with Sodium lactate(100mM) for 24h. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.WB
Western blot analysis of lysates from NIH/3T3 cells, using Lactic acid -Histone H4-K5 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated with Sodium lactate(100mM) for 24h. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.WB
Western blot analysis of lysates from C6 cells, using Lactic acid -Histone H4-K5 Rabbit pAb at 1:1000 dilution. C6 cells were treated with Sodium lactate(100mM) for 24h. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.DB
Dot-blot analysis of all sorts of peptides using Lactic acid -Histone H4-K5 antibody at 1:4000 dilution.| Product Name | Lactic acid -Histone H4-K5 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | DBWB |
| WB:1:500-1:2000 DB:1:500-1:4000 |
|
| Species Reactivity | HumanMouseOtherRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H4C1 |
|---|---|
| Gene Synonyms | H4FA HIST1H4A |
| Gene Full Name | H4 clustered histone 1 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 11 kDa(Observed band size: 14 kDa) |
| Cellular Localization | extracellular exosome, extracellular region, nucleoplasm, nucleus. |
| SwissProt ID | P62805 |
|---|
WB
Western blot analysis of lysates from MCF7 cells, using Lactic acid -Histone H4-K5 Rabbit pAb at 1:1000 dilution. MCF7 cells were treated with Sodium lactate(100mM) for 24h. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
WB
Western blot analysis of lysates from NIH/3T3 cells, using Lactic acid -Histone H4-K5 Rabbit pAb at 1:1000 dilution. NIH/3T3 cells were treated with Sodium lactate(100mM) for 24h. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
WB
Western blot analysis of lysates from C6 cells, using Lactic acid -Histone H4-K5 Rabbit pAb at 1:1000 dilution. C6 cells were treated with Sodium lactate(100mM) for 24h. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 60s.
DB
Dot-blot analysis of all sorts of peptides using Lactic acid -Histone H4-K5 antibody at 1:4000 dilution.| Application Notes | WB:1:500-1:2000 DB:1:500-1:4000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
