IHC
Immunohistochemistry analysis of paraffin-embedded Rat ovary using Acetyl-Histone H1.4-K26 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Acetyl-Histone H1.4-K26 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.IHC
Immunohistochemistry analysis of paraffin-embedded Mouse brain using Acetyl-Histone H1.4-K26 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.ICC/IF
Immunofluorescence analysis of C6 cells using Acetyl-Histone H1.4-K26 at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H1.4-K26 at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.ICC/IF
Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H1.4-K26 at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.U2OS cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Product Name | Acetyl-Histone H1.4-K26 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC |
| IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H1-4 |
|---|---|
| Gene Synonyms | H1E H1.4 H1F4 RMNS H1s-4 HIST1H1E dJ221C16.5 |
| Gene Full Name | H1.4 linker histone, cluster member |
| Gene Summary | Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 22 kDa |
| Cellular Localization | nucleus. |
| SwissProt ID | P10412 |
|---|
IHC
Immunohistochemistry analysis of paraffin-embedded Rat ovary using Acetyl-Histone H1.4-K26 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Human tonsil using Acetyl-Histone H1.4-K26 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
IHC
Immunohistochemistry analysis of paraffin-embedded Mouse brain using Acetyl-Histone H1.4-K26 Rabbit pAb at dilution of 1:200 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ICC/IF
Immunofluorescence analysis of C6 cells using Acetyl-Histone H1.4-K26 at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.C6 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H1.4-K26 at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.NIH/3T3 cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
ICC/IF
Immunofluorescence analysis of U-2 OS cells using Acetyl-Histone H1.4-K26 at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.U2OS cells were treated by TSA (1 uM) at 37℃ for 18 hours. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.| Application Notes | IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
