WB
Western blot analysis of various lysates using Acetyl-Histone H2B-K20 Rabbit pAb at 1:500 dilution. HeLa cells,NIH/3T3 cells and C6 cells were treated with TSA (1 μM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.CHIP
Chromatin immunoprecipitation was performed with cross-linked chromatin from 293F cells, using Acetyl-Histone H2B-K20 Rabbit pAb and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram compares the ratio of the immunoprecipitated DNA versus the input.| Product Name | Acetyl-Histone H2B-K20 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ChIPWB |
| WB:1:500-1:1000 ChIP:5μg antibody for 5μg-10μg of Chromatin |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | H2BC5 |
|---|---|
| Gene Synonyms | H2B/a H2B/b H2B/g H2B/h H2B/k H2B/l H2BFA H2BFB H2BFG H2BFH H2BFK H2BFL H2B.1B HIRIP2 HIST1H2BC HIST1H2BD HIST1H2BE HIST1H2BF HIST1H2BG HIST1H2BI dJ221C16.6 |
| Gene Full Name | H2B clustered histone 5 |
| Gene Summary | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H2B family. Two transcripts that encode the same protein have been identified for this gene, which is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Aug 2015] |
| Molecular Weight(MW) | 14 kDa(Observed band size: 17 kDa) |
| Cellular Localization | Chromosome, Nucleus. |
| SwissProt ID | P62807 |
|---|
WB
Western blot analysis of various lysates using Acetyl-Histone H2B-K20 Rabbit pAb at 1:500 dilution. HeLa cells,NIH/3T3 cells and C6 cells were treated with TSA (1 μM) at 37℃ for 18 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.
CHIP
Chromatin immunoprecipitation was performed with cross-linked chromatin from 293F cells, using Acetyl-Histone H2B-K20 Rabbit pAb and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram compares the ratio of the immunoprecipitated DNA versus the input.| Application Notes | WB:1:500-1:1000 ChIP:5μg antibody for 5μg-10μg of Chromatin |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
