WB
Western blot analysis of anti-PSIP1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PSIP1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of PSIP1 using anti-PSIP1 antibody. PSIP1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-PSIP1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of PSIP1 using anti-PSIP1 antibody. PSIP1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-PSIP1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of PSIP1 using anti-PSIP1 antibody. PSIP1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-PSIP1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of PSIP1 using anti-PSIP1 antibody and anti-Beta Tubulin antibody. PSIP1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-PSIP1 Antibody at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (Green) were used as secondary antibody.FC
Flow Cytometry analysis of K562 cells using anti-PSIP1 antibody. Overlay histogram showing K562 cells stained with anti-PSIP1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSIP1 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to PSIP1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human PSIP1 recombinant protein (Position: D280-N530). Human PSIP1 shares 92.8% and 91.2% amino acid (aa) sequence identity with mouse and rat PSIP1, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PSIP1 |
|---|---|
| Gene Synonyms | p52 p75 PAIP DFS70 LEDGF PSIP2 |
| Gene Full Name | PC4 and SRSF1 interacting protein 1 |
| Gene Summary | Enables DNA-binding transcription factor binding activity; chromatin binding activity; and transcription coactivator activity. Involved in mRNA 5'-splice site recognition and positive regulation of transcription by RNA polymerase II. Located in heterochromatin; nuclear periphery; and nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | CLL associated antigen KW 7 DFS 70 DFS70 LEDGF p52 p75 PAIP PSIP1 PSIP2 |
| Molecular Weight(MW) | 60 kDa(Observed band size: 75-85 kDa) |
| Cellular Localization | Nucleus |
WB
Western blot analysis of anti-PSIP1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PSIP1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of PSIP1 using anti-PSIP1 antibody. PSIP1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-PSIP1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of PSIP1 using anti-PSIP1 antibody. PSIP1 was detected in a paraffin-embedded section of mouse brain tissue. The tissue section was incubated with rabbit anti-PSIP1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of PSIP1 using anti-PSIP1 antibody. PSIP1 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-PSIP1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of PSIP1 using anti-PSIP1 antibody and anti-Beta Tubulin antibody. PSIP1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-PSIP1 Antibody at a dilution of 1:100. Cy3-Conjugated Anti-rabbit IgG Secondary Antibody (Red) and Fluoro488-conjugated Anti-mouse IgG Secondary Antibody (Green) were used as secondary antibody.
FC
Flow Cytometry analysis of K562 cells using anti-PSIP1 antibody. Overlay histogram showing K562 cells stained with anti-PSIP1 antibody (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PSIP1 Antibody (1:100). Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
