WB
Western Blot analysis of SH-SY5Y cells using Phospho-IRS-1 (S636) Polyclonal Antibody diluted at 1:2000.WB
Western blot analysis of lysates from 293 cells and COLO205 cells, using IRS-1 (Phospho-Ser636) Antibody. The lane on the right is blocked with the phospho peptide.IHC
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4℃ overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.IHC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IRS-1 (Phospho-Ser636) Antibody. The picture on the right is blocked with the phospho peptide.ICC/IF
Immunofluorescence analysis of MCF7 cells, using IRS-1 (Phospho-Ser636) Antibody. The picture on the right is blocked with the phospho peptide.| Product Name | IRS-1 (Phospho Ser636) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IRS-1 around the phosphorylation site of Ser636. AA range:603-652 |
| Modification | p-Ser636 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IRS1 |
|---|---|
| Gene Synonyms | HIRS-1 |
| Gene Full Name | insulin receptor substrate 1 |
| Gene Summary | This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009] |
| Alternative Names | IRS1 Insulin receptor substrate 1 IRS-1 |
| Molecular Weight(MW) | 170 kDa |
| Cellular Localization | nucleus ,cytoplasm ,cytosol ,plasma membrane ,insulin receptor complex ,caveola ,intracellular membrane-bounded organelle, |
WB
Western Blot analysis of SH-SY5Y cells using Phospho-IRS-1 (S636) Polyclonal Antibody diluted at 1:2000.
WB
Western blot analysis of lysates from 293 cells and COLO205 cells, using IRS-1 (Phospho-Ser636) Antibody. The lane on the right is blocked with the phospho peptide.
IHC
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4℃ overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
IHC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IRS-1 (Phospho-Ser636) Antibody. The picture on the right is blocked with the phospho peptide.
ICC/IF
Immunofluorescence analysis of MCF7 cells, using IRS-1 (Phospho-Ser636) Antibody. The picture on the right is blocked with the phospho peptide.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
