WB
Western blot (WB) analysis of p-IRS-1 (Y612) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:A375 whole cell lysate(40ug) Lane4:A2780 whole cell lysate(40ug) Lane5:Hela whole cell lysate(40ug) Lane6:C6 whole cell lysate(40ug) Lane7:NIH-3T3 whole cell lysate(40ug).IHC
Immunohistochemistry (IHC) analyzes of p-IRS-1 (Y612) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.| Product Name | IRS-1 (phospho-Y612) polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | p-IRS-1 (Y612) polyclonal antibody detects endogenous levels of IRS-1 protein only when phosphorylated at Ser612. |
| Modification | p-Y612 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:1000 IHC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | IRS1 |
|---|---|
| Gene Synonyms | HIRS-1 |
| Gene Full Name | insulin receptor substrate 1 |
| Gene Summary | This gene encodes a protein which is phosphorylated by insulin receptor tyrosine kinase. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. [provided by RefSeq, Nov 2009] |
| Molecular Weight(MW) | 180 KDa |
| Cellular Localization | Cytoplasm,Nucleus |
WB
Western blot (WB) analysis of p-IRS-1 (Y612) pAb at 1:500 dilution Lane1:MCF-7 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:A375 whole cell lysate(40ug) Lane4:A2780 whole cell lysate(40ug) Lane5:Hela whole cell lysate(40ug) Lane6:C6 whole cell lysate(40ug) Lane7:NIH-3T3 whole cell lysate(40ug).
IHC
Immunohistochemistry (IHC) analyzes of p-IRS-1 (Y612) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.| Application Notes | WB:1:500-1:1000 IHC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 |
