WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃ over night with a 1:500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.WB
Various whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-BRCA1 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.WB
Western Blot analysis of various cells using BRCA1 Antibody diluted at 1:1000.IHC
Human tonsil was stained with anti-BRCA1 rabbit antibody.WB
Bao, K., Li, Y., Wei, J. et al. Fangchinoline suppresses conjunctival melanoma by directly binding FUBP2 and inhibiting the homologous recombination pathway. Cell Death Dis 12, 380 (2021).ICC/IF
Immunofluorescence analysis of Hela cell. 1,mouse Tubulin Antibody (green) was diluted at 1:200 (4℃ overnight). BRCA1 Rabbit mAb (green) was diluted at 1:200 (4℃ overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).ICC/IF
Immunofluorescence analysis of human-stomach tissue. 1,BRCA1 Antibody (red) was diluted at 1:200 (4℃ overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.| Product Name | BRCA1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthesized peptide derived from C-ternal BRCA1 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| IHC:1:100-1:500 WB:1:10000-1:50000 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | BRCA1 |
|---|---|
| Gene Synonyms | IRIS PSCP BRCAI BRCC1 FANCS PNCA4 RNF53 BROVCA1 PPP1R53 |
| Gene Full Name | BRCA1 DNA repair associated |
| Gene Summary | This gene encodes a 190 kD nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The BRCA1 gene contains 22 exons spanning about 110 kb of DNA. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2020] |
| Alternative Names | BRCA1 RNF53 Breast cancer type 1 susceptibility protein RING finger protein 53 |
| Molecular Weight(MW) | 208 kDa(Observed band size: 270 kDa) |
| Cellular Localization | Nucleus . Chromosome . Cytoplasm . Localizes at sites of DNA damage at double-strand breaks (DSBs) ; recruitment to DNA damage sites is mediated by ABRAXAS1 and the BRCA1-A complex (PubMed:26778126) . Translocated to the cytoplasm during UV-induced apoptosis (PubMed:20160719) . .; [Isoform 3]: Cytoplasm.; [Isoform 5]: Cytoplasm . |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃ over night with a 1:500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.
WB
Various whole cell lysates were separated by 4-8% SDS-PAGE, and the membrane was blotted with anti-BRCA1 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
WB
Western Blot analysis of various cells using BRCA1 Antibody diluted at 1:1000.
IHC
Human tonsil was stained with anti-BRCA1 rabbit antibody.
WB
Bao, K., Li, Y., Wei, J. et al. Fangchinoline suppresses conjunctival melanoma by directly binding FUBP2 and inhibiting the homologous recombination pathway. Cell Death Dis 12, 380 (2021).
ICC/IF
Immunofluorescence analysis of Hela cell. 1,mouse Tubulin Antibody (green) was diluted at 1:200 (4℃ overnight). BRCA1 Rabbit mAb (green) was diluted at 1:200 (4℃ overnight). 2, Goat Anti Rabbit Alexa Fluor 594 was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 was diluted at 1:1000 (room temperature, 50min).
ICC/IF
Immunofluorescence analysis of human-stomach tissue. 1,BRCA1 Antibody (red) was diluted at 1:200 (4℃ overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.| Application Notes | IHC:1:100-1:500 WB:1:10000-1:50000 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
