WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NLRP3 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.WB
Western blot analysis of lysate from K562 cells, using NLRP3 Antibody.WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃ over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.IHC
Rat kidney tissue was stained with Anti-NLRP3 rabbit Antibody.| Product Name | NLRP3 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| IHC:1:200-1000 WB:1:500-5000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NLRP3 |
|---|---|
| Gene Synonyms | AII AVP FCU MWS FCAS KEFH CIAS1 FCAS1 NALP3 C1orf7 CLR1.1 DFNA34 PYPAF1 AGTAVPRL |
| Gene Full Name | NLR family pyrin domain containing 3 |
| Gene Summary | This gene encodes a pyrin-like protein containing a pyrin domain, a nucleotide-binding site (NBS) domain, and a leucine-rich repeat (LRR) motif. This protein interacts with the apoptosis-associated speck-like protein PYCARD/ASC, which contains a caspase recruitment domain, and is a member of the NLRP3 inflammasome complex. This complex functions as an upstream activator of NF-kappaB signaling, and it plays a role in the regulation of inflammation, the immune response, and apoptosis. The SARS-CoV 3a protein, a transmembrane pore-forming viroporin, has been shown to activate the NLRP3 inflammasome via the formation of ion channels in macrophages. Mutations in this gene are associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, neonatal-onset multisystem inflammatory disease (NOMID), keratoendotheliitis fugax hereditarian, and deafness, autosomal dominant 34, with or without inflammation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. Alternative 5' UTR structures are suggested by available data; however, insufficient evidence is available to determine if all of the represented 5' UTR splice patterns are biologically valid. [provided by RefSeq, Aug 2020] |
| Alternative Names | NLRP3 C1orf7 CIAS1 NALP3 PYPAF1 NACHT LRR and PYD domains-containing protein 3 Angiotensin/vasopressin receptor AII/AVP-like Caterpiller protein 1.1CLR1.1 Cold autoinflammatory syndrome 1 protein Cryopyrin PYRIN-containing APAF1-like protein 1 |
| Molecular Weight(MW) | 118 kDa |
| Cellular Localization | Cytoplasm , Nuclear |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-NLRP3 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
WB
Western blot analysis of lysate from K562 cells, using NLRP3 Antibody.
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃ over night with a 1:2500 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.
IHC
Rat kidney tissue was stained with Anti-NLRP3 rabbit Antibody.| Application Notes | IHC:1:200-1000 WB:1:500-5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
