WB
Western Blot analysis of 3T3 cells using Phospho-IL-8Rβ (S347) Polyclonal AntibodyWB
Western blot analysis of lysates from NIH/3T3 cells treated with PMA 125ng/ml 30', using IL-8R beta/CDw128 beta (Phospho-Ser347) Antibody. The lane on the right is blocked with the phospho peptide.IHC
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4℃ overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.IHC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IL-8R beta/CDw128 beta (Phospho-Ser347) Antibody. The picture on the right is blocked with the phospho peptide.ICC/IF
Immunofluorescence analysis of COS7 cells, using IL-8R beta/CDw128 beta (Phospho-Ser347) Antibody. The picture on the right is blocked with the phospho peptide.| Product Name | IL-8Rβ (Phospho Ser347) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IL-8R beta/CDw128 beta around the phosphorylation site of Ser347. AA range:311-360 |
| Modification | p-Ser347 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CXCR2 |
|---|---|
| Gene Synonyms | CD182 IL8R2 IL8RA IL8RB SCN13 CMKAR2 WHIMS2 CDw128b |
| Gene Full Name | C-X-C motif chemokine receptor 2 |
| Gene Summary | The protein encoded by this gene is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Nov 2009] |
| Alternative Names | CXCR2 IL8RB C-X-C chemokine receptor type 2 CXC-R2 CXCR-2 CDw128b GRO/MGSA receptor High affinity interleukin-8 receptor B IL-8R B IL-8 receptor type 2 CD antigen CD182 |
| Molecular Weight(MW) | 48 kDa |
| Cellular Localization | Cell membrane; Multi-pass membrane protein. |
WB
Western Blot analysis of 3T3 cells using Phospho-IL-8Rβ (S347) Polyclonal Antibody
WB
Western blot analysis of lysates from NIH/3T3 cells treated with PMA 125ng/ml 30', using IL-8R beta/CDw128 beta (Phospho-Ser347) Antibody. The lane on the right is blocked with the phospho peptide.
IHC
Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4℃ overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
IHC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IL-8R beta/CDw128 beta (Phospho-Ser347) Antibody. The picture on the right is blocked with the phospho peptide.
ICC/IF
Immunofluorescence analysis of COS7 cells, using IL-8R beta/CDw128 beta (Phospho-Ser347) Antibody. The picture on the right is blocked with the phospho peptide.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
