raised against a
thymic reticulum of mouse origin.
Key Feature
Clonality
Monoclonal
Isotype
IgG2a
Host Species
Rat
Tested Applications
WB
WB:1:100-1:1000
Species Reactivity
HumanMouseRat
Concentration
0.1mg/ml
Purification
Affinity purified
Target Information
Function
Fibroblast cells synthesize and maintain the extracellular matrix in a wide
variety of mammal tissues. Fibroblasts provide a structural framework for
many tissues and also play an important role in wound healing. They are con
tinuously secreting precursors of the extracellular matrix, specifically the col
lagens, glycosaminoglycans, reticular and elastic fibers and glycoproteins.
Fibroblasts are morphologically heterogeneous and are not restricted by a
polarizing attachment to a basal lamina. Fibroblasts have a branched cyto
plasm surrounding an elliptical, speckled nucleus having one or two nucleoli.
Fibroblasts proliferate easily, making them a popular cell type for cell cultures
in biological research. Fibroblast markers can aid in the identification and
behavioral analysis of these cells. The intermediate filament protein vimentin,
for example, is expressed on Fibroblast cells, and it is used as a marker to
distinguish the mesodermal origin of the cells.
Application
WB
FibroblastMarker HRP:Rat Monoclonal antibody to Fibroblast Marker.Direct
westernblotanalysisofFibroblastMarkerexpression
in3611-RFwholecell lysate.
Application Notes
WB:1:100-1:1000
Additional Information
Form
Liquid
Storage Instructions
Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of
shipment. Non-hazardous. No MSDS required.
Storage Buffer
Each vial contains 200 µg IgG2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.