WB
Relative expression (RE) Western blot analysis of Granzyme K on different lysates with Rabbit anti-Granzyme K antibody at 1/5,000 dilution. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human NK/T lymphoma tissue with Rabbit anti-Granzyme K antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IF-P
Application: IF-Tissue Species: Human Site: NK/T lymphoma Sample: Paraffin-embedded section Antibody concentration: 1/500| Product Name | Granzyme K Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human Granzyme K aa 1-264. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IF-PIHCWB |
| WB:1:5000 IHC:1:1000 IF-P:1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | GZMK |
|---|---|
| Gene Synonyms | GrK TRYP2 |
| Gene Full Name | granzyme K |
| Gene Summary | This gene product is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes. [provided by RefSeq, Jul 2008] |
| Alternative Names | Fragmentin 3 antibody Fragmentin-3 antibody Fragmentin3 antibody GRAK_HUMAN antibody Granzyme 3 antibody granzyme K (granzyme 3 tryptase II) antibody granzyme K (serine protease granzyme 3 tryptase II) antibody Granzyme K antibody Fragmentin 3 antibody Fragmentin-3 antibody Fragmentin3 antibody GRAK_HUMAN antibody Granzyme 3 antibody granzyme K (granzyme 3 tryptase II) antibody granzyme K (serine protease granzyme 3 tryptase II) antibody Granzyme K antibody Granzyme K precursor antibody Granzyme-3 antibody Granzyme3 antibody GranzymeK antibody GZMK antibody NK TRYP 2 antibody NK TRYP2 antibody NK tryptase 2 antibody NK-TRYP-2 antibody NK-tryptase-2 antibody NKTRYP2 antibody Serine protease granzyme 3 antibody TRYP 2 antibody TRYP2 antibody Tryptase II antibody TryptaseII antibody |
| Molecular Weight(MW) | 29 kDa(Observed band size: 27 kDa) |
| Cellular Localization | Secreted, Cytoplasmic granule. |
WB
Relative expression (RE) Western blot analysis of Granzyme K on different lysates with Rabbit anti-Granzyme K antibody at 1/5,000 dilution. Exposure time: 10 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/5,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human NK/T lymphoma tissue with Rabbit anti-Granzyme K antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IF-P
Application: IF-Tissue Species: Human Site: NK/T lymphoma Sample: Paraffin-embedded section Antibody concentration: 1/500| Application Notes | WB:1:5000 IHC:1:1000 IF-P:1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
