WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CHOP antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human breast carcinoma was stained with anti-CHOP Rabbit antibody.ICC/IF
Immunofluorescence analysis of HEK293. Picture A: CHOP antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Product Name | CHOP Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:5000 IHC:1:100-1:400 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | ChickenHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | DDIT3 |
|---|---|
| Gene Synonyms | DDIT3 |
| Gene Full Name | DNA damage inducible transcript 3 |
| Gene Summary | This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified. [provided by RefSeq, Aug 2010] |
| Alternative Names | DDIT3 |
| Molecular Weight(MW) | 19 kDa(Observed band size: 30 kDa) |
| Cellular Localization | Cytoplasm,Nucleus. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-CHOP antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human breast carcinoma was stained with anti-CHOP Rabbit antibody.
ICC/IF
Immunofluorescence analysis of HEK293. Picture A: CHOP antibody (red). Picture B: DAPI (blue). Picture C: Merge of A+B.| Application Notes | WB:1:1000-1:5000 IHC:1:100-1:400 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C) |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA |
