WB
Western blot analysis of Insulin R (phospho Tyr1361) Polyclonal Antibody, using hela,293 cell treated or untreated with EGF 200ng/ml 30', 4℃ over night, secondary antibody at 1:10000 dilution, incubated at 37℃ for 1 hour..IHC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IR (Phospho-Tyr1361) Antibody. The picture on the right is blocked with the phospho peptide.| Product Name | Insulin R (Phospho Tyr1361) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human IR around the phosphorylation site of Tyr1361. AA range:1331-1380. |
| Modification | p-Tyr1361 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | INSR |
|---|---|
| Gene Synonyms | HHF5 CD220 |
| Gene Full Name | insulin receptor |
| Gene Summary | This gene encodes a member of the receptor tyrosine kinase family of proteins. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that form a heterotetrameric receptor. Binding of insulin or other ligands to this receptor activates the insulin signaling pathway, which regulates glucose uptake and release, as well as the synthesis and storage of carbohydrates, lipids and protein. Mutations in this gene underlie the inherited severe insulin resistance syndromes including type A insulin resistance syndrome, Donohue syndrome and Rabson-Mendenhall syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015] |
| Alternative Names | INSR Insulin receptor IR CD antigen CD220 |
| Molecular Weight(MW) | 95kDa |
| Cellular Localization | Cell membrane,Endosome,Lysosome,Membrane. |
WB
Western blot analysis of Insulin R (phospho Tyr1361) Polyclonal Antibody, using hela,293 cell treated or untreated with EGF 200ng/ml 30', 4℃ over night, secondary antibody at 1:10000 dilution, incubated at 37℃ for 1 hour..
IHC
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using IR (Phospho-Tyr1361) Antibody. The picture on the right is blocked with the phospho peptide.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year(Do not lower than -25°C) |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
