WB
Western blot analysis of S100A9 using anti-S100A9 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-S100A9 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of S100A9 using anti-S100A9 antibody. S100A9 was detected in a paraffin-embedded section of appendicitis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-S100A9 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IF-P
IF analysis using Anti-S100A9 antibody detected in paraffin-embedded section of human Lung cancer tissue. The tissue section were stained using the Fluoro488 conjugated Anti-rabbit IgG Secondary Antibody (green) and counterstained with DAPI (blue).ICC/IF
ICC/IF analysis of S100A9 using anti-S100A9 antibody. S100A9 was detected in an immunocytochemical section of SKOV cells. The section was incubated with rabbit anti-S100A9 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).FC
Flow Cytometry analysis of A431 cells using anti-S100A9 antibody. Overlay histogram showing A431 cells stained with Rabbit polyclonal antibody to S100A9 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-S100A9 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to S100A9 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived human S100A9 recombinant protein (Position: T2-P114). Human S100A9 shares 59.8% and 64.5% amino acid (aa) sequence identity with mouse and rat S100A9, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | S100A9 |
|---|---|
| Gene Synonyms | MIF NIF P14 CAGB CFAG CGLB L1AG LIAG MRP14 60B8AG MAC387 S100-A9 |
| Gene Full Name | S100 calcium binding protein A9 |
| Gene Summary | The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and altered expression of this protein is associated with the disease cystic fibrosis. This antimicrobial protein exhibits antifungal and antibacterial activity. [provided by RefSeq, Nov 2014] |
| Alternative Names | 60B8AG CAGB Calgranulin B Calprotectin L1H subunit CFAG CGLB L1AG LIAG MAC387 MIF MRP 14 MRP14 NIF P14 Protein S100 A9 S100A9 |
| Molecular Weight(MW) | 13 kDa |
| Cellular Localization | Cell membrane,Cytoplasm,Cytoskeleton,Membrane,Secreted. |
WB
Western blot analysis of S100A9 using anti-S100A9 antibody . The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-S100A9 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of S100A9 using anti-S100A9 antibody. S100A9 was detected in a paraffin-embedded section of appendicitis tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-S100A9 Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IF-P
IF analysis using Anti-S100A9 antibody detected in paraffin-embedded section of human Lung cancer tissue. The tissue section were stained using the Fluoro488 conjugated Anti-rabbit IgG Secondary Antibody (green) and counterstained with DAPI (blue).
ICC/IF
ICC/IF analysis of S100A9 using anti-S100A9 antibody. S100A9 was detected in an immunocytochemical section of SKOV cells. The section was incubated with rabbit anti-S100A9 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).
FC
Flow Cytometry analysis of A431 cells using anti-S100A9 antibody. Overlay histogram showing A431 cells stained with Rabbit polyclonal antibody to S100A9 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-S100A9 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IF-P:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
