WB
Cell treatment (CT) Western blot analysis of Phospho-EGFR (Y845) on different lysates with Rabbit anti-Phospho-EGFR (Y845) antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.| Product Name | Phospho-EGFR (Y845) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Tyr845 of human EGFR protein. |
| Modification | p-Y845 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | EGFR |
|---|---|
| Gene Synonyms | ERBB ERRP HER1 mENA ERBB1 NNCIS PIG61 NISBD2 |
| Gene Full Name | epidermal growth factor receptor |
| Gene Summary | The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor, thus inducing receptor dimerization and tyrosine autophosphorylation leading to cell proliferation. Mutations in this gene are associated with lung cancer. EGFR is a component of the cytokine storm which contributes to a severe form of Coronavirus Disease 2019 (COVID-19) resulting from infection with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). [provided by RefSeq, Jul 2020] |
| Alternative Names | Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody Cell growth inhibiting protein 40 antibody Cell proliferation inducing protein 61 antibody EGF R antibody EGFR antibody EGFR_HUMAN antibody Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody Epidermal growth factor receptor antibody erb-b2 receptor tyrosine kinase 1 antibody Avian erythroblastic leukemia viral (v erb b) oncogene homolog antibody Cell growth inhibiting protein 40 antibody Cell proliferation inducing protein 61 antibody EGF R antibody EGFR antibody EGFR_HUMAN antibody Epidermal growth factor receptor (avian erythroblastic leukemia viral (v erb b) oncogene homolog) antibody Epidermal growth factor receptor (erythroblastic leukemia viral (v erb b) oncogene homolog avian) antibody Epidermal growth factor receptor antibody erb-b2 receptor tyrosine kinase 1 antibody ERBB antibody ERBB1 antibody Errp antibody HER1 antibody mENA antibody NISBD2 antibody Oncogen ERBB antibody PIG61 antibody Proto-oncogene c-ErbB-1 antibody Receptor tyrosine protein kinase ErbB 1 antibody Receptor tyrosine-protein kinase ErbB-1 antibody SA7 antibody Species antigen 7 antibody Urogastrone antibody v-erb-b Avian erythroblastic leukemia viral oncogen homolog antibody wa2 antibody Wa5 antibody |
| Molecular Weight(MW) | 134 kDa(Observed band size: 180/75 kDa) |
| Cellular Localization | Cell membrane, Nucleus membrane, Nucleus, Endoplasmic reticulum membrane, Golgi apparatus membrane, Endosome. |
WB
Cell treatment (CT) Western blot analysis of Phospho-EGFR (Y845) on different lysates with Rabbit anti-Phospho-EGFR (Y845) antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
