WB
Western blot analysis of HNF4A using anti-HNF4A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HNF4A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of HNF4A using anti-HNF4A antibody. HNF4A was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-HNF4A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of HNF4A using anti-HNF4A antibody. HNF4A was detected in a paraffin-embedded section of mouse intestine tissue. The tissue section was incubated with rabbit anti-HNF4A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of HNF4A using anti-HNF4A antibody. HNF4A was detected in a paraffin-embedded section of rat liver tissue. The tissue section was incubated with rabbit anti-HNF4A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of HNF-4-alpha using anti- HNF-4-alpha antibody.HNF-4-alpha was detected in immunocytochemical section of HEPG2 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti- HNF-4-alpha Antibody overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.FC
Flow Cytometry analysis of HEPG2 cells using anti- HNF-4-alpha antibody.Overlay histogram showing HEPG2 cells stained with PB9215 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HNF-4-alpha Antibody (1:100) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to HNF4A |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human HNF-4-alpha recombinant protein (Position: Q164-I474). Human HNF-4-alpha shares 95% and 96% amino acid (aa) sequences identity with mouse and rat HNF-4-alpha, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | HNF4A |
|---|---|
| Gene Synonyms | TCF HNF4 MODY FRTS4 MODY1 NR2A1 TCF14 HNF4a7 HNF4a8 HNF4a9 NR2A21 TCF-14 HNF4alpha |
| Gene Full Name | hepatocyte nuclear factor 4 alpha |
| Gene Summary | The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012] |
| Alternative Names | hepatocyte nuclear factor 4 alpha FRTS4 HNF4 HNF4a7 HNF4a8 HNF4a9 HNF4alpha MODY MODY1 NR2A1 NR2A21 TCF TCF14 HNF4A |
| Molecular Weight(MW) | 53 kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of HNF4A using anti-HNF4A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HNF4A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of HNF4A using anti-HNF4A antibody. HNF4A was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-HNF4A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of HNF4A using anti-HNF4A antibody. HNF4A was detected in a paraffin-embedded section of mouse intestine tissue. The tissue section was incubated with rabbit anti-HNF4A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of HNF4A using anti-HNF4A antibody. HNF4A was detected in a paraffin-embedded section of rat liver tissue. The tissue section was incubated with rabbit anti-HNF4A Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of HNF-4-alpha using anti- HNF-4-alpha antibody.HNF-4-alpha was detected in immunocytochemical section of HEPG2 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL rabbit anti- HNF-4-alpha Antibody overnight at 4°C. Fluoro488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
FC
Flow Cytometry analysis of HEPG2 cells using anti- HNF-4-alpha antibody.Overlay histogram showing HEPG2 cells stained with PB9215 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HNF-4-alpha Antibody (1:100) for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
