Western Blot
Positive WB detected in: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E. Coli
SARS-CoV-2 nucleocapsid antibody at 1:1000
Secondary
Peroxidase-Affinipure Goat Anti-Human IgG Fc Fragment Specific at 1/20000 dilution.
Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa)
Key Feature
Clonality
Monoclonal
Isotype
IgG1
Tested Applications
WB
WB:1:500-1:5000
Species Reactivity
Virus
Concentration
1mg/ml
Target Information
Alternative Names
N Nucleoprotein N Nucleocapsid protein NC Protein N
Molecular Weight(MW)
48 kDa(Observed band size: 55 kDa)
Function
Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
Cellular Localization
Host cytoplasm,Secreted,Virion.
Application
WB
Western Blot
Positive WB detected in: His tag-tagged SARS-CoV-2 nucleocapsid recombinant protein from E. Coli
SARS-CoV-2 nucleocapsid antibody at 1:1000
Secondary
Peroxidase-Affinipure Goat Anti-Human IgG Fc Fragment Specific at 1/20000 dilution.
Application Notes
WB:1:500-1:5000
Additional Information
Form
Liquid
Storage Instructions
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.