WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃ over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.WB
Western blot analysis of lysates from 1) mouse-brain , 2) mouse-lung, 3) Hela cells, (Green) primary antibody was diluted at 1:1000, 4℃ over night, secondary antibody was diluted at 1:10000, 37° 1hour. (Red) GAPDH Monoclonal Antibody antibody was diluted at 1:5000 as loading control, 4℃ over night,secondary antibody was diluted at 1:10000, 37° 1hour.WB
Western blot analysis of lysates from Jurkat WT and knockdown cell. Cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with Beclin 1 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Rat kidney was stained with anti-Beclin 1 rabbit antibody.IHC
Mouse kidney was stained with anti-Beclin 1 rabbit antibody.| Product Name | Beclin 1 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000-1:10000 IHC:1:200-1:1000 |
|
| Species Reactivity | ChickenHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | BECN1 |
|---|---|
| Gene Synonyms | ATG6 VPS30 beclin1 |
| Gene Full Name | beclin 1 |
| Gene Summary | This gene encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015] |
| Alternative Names | Beclin-1 (Coiled-coil myosin-like BCL2-interacting protein) (Protein GT197) |
| Molecular Weight(MW) | 52 kDa(Observed band size: 60 kDa) |
| Cellular Localization | Cytoplasm. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the primary antibody was used at 4℃ over night with a 1:5000 dilution . The Dylight 800-conjugated Goat anti-Rabbit antibody was used to detect the antibody.
WB
Western blot analysis of lysates from 1) mouse-brain , 2) mouse-lung, 3) Hela cells, (Green) primary antibody was diluted at 1:1000, 4℃ over night, secondary antibody was diluted at 1:10000, 37° 1hour. (Red) GAPDH Monoclonal Antibody antibody was diluted at 1:5000 as loading control, 4℃ over night,secondary antibody was diluted at 1:10000, 37° 1hour.
WB
Western blot analysis of lysates from Jurkat WT and knockdown cell. Cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with Beclin 1 Rabbit mAb. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Rat kidney was stained with anti-Beclin 1 rabbit antibody.
IHC
Mouse kidney was stained with anti-Beclin 1 rabbit antibody.| Application Notes | WB:1:2000-1:10000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
