WB
Western blot analysis of TFG using anti-TFG antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TFG antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of TFG using anti-TFG antibody. TFG was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-TFG Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of TFG using anti-TFG antibody. TFG was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-TFG Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of TFG using anti-TFG antibody. TFG was detected in a paraffin-embedded section of rat colon tissue. The tissue section was incubated with rabbit anti-TFG Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IF-P
IF analysis using anti- TFG antibody. detected in paraffin-embedded section of human colon cancer tissue. The tissue section were stained using the Fluoro550-conjugated Anti-rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue).ICC/IF
ICC/IF analysis of TFG using anti-TFG antibody. TFG was detected in an immunocytochemical section of U87 cells. The section was incubated with rabbit anti-TFG Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Product Name | Rabbit polyclonal antibody to TFG |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human TRK fused gene/TFG recombinant protein (Position: D121-R400). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-PIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 ICC/IF:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TFG |
|---|---|
| Gene Synonyms | TF6 HMSNP SPG57 TRKT3 |
| Gene Full Name | trafficking from ER to golgi regulator |
| Gene Summary | There are several documented fusion oncoproteins encoded partially by this gene. This gene also participates in several oncogenic rearrangements resulting in anaplastic lymphoma and mixoid chondrosarcoma, and may play a role in the NF-kappaB pathway. Multiple transcript variants have been found for this gene. [provided by RefSeq, Sep 2010] |
| Alternative Names | Protein TFG TF6 TFG TRK fused gene TRK fused gene protein TRKT3 |
| Molecular Weight(MW) | 43 kDa(Observed band size: 60 kDa) |
| Cellular Localization | Endoplasmic reticulum |
WB
Western blot analysis of TFG using anti-TFG antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TFG antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of TFG using anti-TFG antibody. TFG was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-TFG Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of TFG using anti-TFG antibody. TFG was detected in a paraffin-embedded section of human ovarian cancer tissue. The tissue section was incubated with rabbit anti-TFG Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of TFG using anti-TFG antibody. TFG was detected in a paraffin-embedded section of rat colon tissue. The tissue section was incubated with rabbit anti-TFG Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IF-P
IF analysis using anti- TFG antibody. detected in paraffin-embedded section of human colon cancer tissue. The tissue section were stained using the Fluoro550-conjugated Anti-rabbit IgG Secondary Antibody (red) and counterstained with DAPI (blue).
ICC/IF
ICC/IF analysis of TFG using anti-TFG antibody. TFG was detected in an immunocytochemical section of U87 cells. The section was incubated with rabbit anti-TFG Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 IF-P:1:50-1:400 ICC/IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
