WB
Western blot analysis of DPF2 using anti-DPF2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DPF2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of DPF2 using anti-DPF2 antibody. DPF2 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-DPF2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of DPF2 using anti-DPF2 antibody. DPF2 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-DPF2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of DPF2 using anti-DPF2 antibody and anti-Alpha Tubulin antibody. DPF2 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-DPF2 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.| Product Name | Rabbit polyclonal antibody to DPF2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human DPF2 recombinant protein (Position: M1-N271). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | DPF2 |
|---|---|
| Gene Synonyms | REQ CSS7 UBID4 ubi-d4 SMARCG2 |
| Gene Full Name | double PHD fingers 2 |
| Gene Summary | The protein encoded by this gene is a member of the d4 domain family, characterized by a zinc finger-like structural motif. This protein functions as a transcription factor which is necessary for the apoptotic response following deprivation of survival factors. It likely serves a regulatory role in rapid hematopoietic cell growth and turnover. This gene is considered a candidate gene for multiple endocrine neoplasia type I, an inherited cancer syndrome involving multiple parathyroid, enteropancreatic, and pituitary tumors. [provided by RefSeq, Jul 2008] |
| Alternative Names | BAF45D BRG1 associated factor 45D BRG1-associated factor 45D Protein requiem REQ |
| Molecular Weight(MW) | 44 kDa(Observed band size: 40 kDa) |
| Cellular Localization | Cytoplasm,Nucleus |
WB
Western blot analysis of DPF2 using anti-DPF2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-DPF2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of DPF2 using anti-DPF2 antibody. DPF2 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-DPF2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of DPF2 using anti-DPF2 antibody. DPF2 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-DPF2 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of DPF2 using anti-DPF2 antibody and anti-Alpha Tubulin antibody. DPF2 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-DPF2 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
