WB
Western blot analysis of CD8A using anti-CD8A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD8A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of CD8A using anti-CD8A antibody. CD8A was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD8A Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.IHC
IHC analysis of CD8A using anti-CD8A antibody. CD8A was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD8A Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.FC
Flow Cytometry analysis of EL-4 cells using anti-CD8 alpha antibody.Overlay histogram showing EL-4 cells stained with anti-CD8 alpha antibody (Blue line).Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Product Name | Rabbit polyclonal antibody to CD8A |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E. coli-derived mouse CD8 alpha recombinant protein (Position: K28-Y196). Mouse CD8 shares 47.9% and 62.3% amino acid (aa) sequence identity with human and rat CD8, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | MouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Cd8a |
|---|---|
| Gene Synonyms | Ly-2 Ly-B Ly-35 Lyt-2 |
| Gene Full Name | CD8 subunit alpha |
| Gene Summary | Enables identical protein binding activity. Acts upstream of or within several processes, including cytotoxic T cell differentiation; defense response to virus; and positive regulation of calcium-mediated signaling. Located in external side of plasma membrane. Part of receptor complex. Is expressed in several structures, including endocrine gland; exocrine gland; genitourinary system; gut; and immune system. Human ortholog(s) of this gene implicated in immunodeficiency 116. Orthologous to human CD8A (CD8 subunit alpha). [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | CD8 CD8A CD8a molecule Leu2 MAL p32 |
| Molecular Weight(MW) | 27 kDa(Observed band size: 26 kDa) |
| Cellular Localization | Cell membrane,Membrane |
WB
Western blot analysis of CD8A using anti-CD8A antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat thymus tissue lysates. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD8A antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of CD8A using anti-CD8A antibody. CD8A was detected in a paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD8A Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC
IHC analysis of CD8A using anti-CD8A antibody. CD8A was detected in a paraffin-embedded section of rat spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-CD8A Antibody at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
FC
Flow Cytometry analysis of EL-4 cells using anti-CD8 alpha antibody.Overlay histogram showing EL-4 cells stained with anti-CD8 alpha antibody (Blue line).Fluoro488 conjugated goat anti-rabbit IgG (1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
