WB
Western blot analysis of MEF2A + MEF2C on THP-1 cell lysate with Rabbit anti-MEF2A + MEF2C antibody at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 46 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-MEF2A + MEF2C antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MEF2A + MEF2C antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-MEF2A + MEF2C antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
Immunocytochemistry analysis of THP-1 cells labeling MEF2A + MEF2C with Rabbit anti-MEF2A + MEF2C antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MEF2A + MEF2C antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (HA1126) was used as the secondary antibody at 1/1,000 dilution.FC
Flow cytometric analysis of Daudi cells labeling MEF2A + MEF2C. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).IP
MEF2A + MEF2C was immunoprecipitated from 0.2 mg THP-1 cell lysate with Recombinant Rabbit Monoclonal Antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Recombinant Rabbit Monoclonal Antibody at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: THP-1 cell lysate (input). Lane 2: Recombinant Rabbit Monoclonal Antibody IP in THP-1 cell lysate. Lane 3: Rabbit IgG instead of Recombinant Rabbit Monoclonal Antibody in THP-1 cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes;| Product Name | MEF2A + MEF2C Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within human MEF2C aa 250-473/473. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFIHCIPWB |
| WB:1:2000 IHC:1:400-1:1000 ICC/IF:1:100 FC:1:500-1:1000 IP:1-2μg/sample |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MEF2A |
|---|---|
| Gene Synonyms | mef2 ADCAD1 RSRFC4 RSRFC9 |
| Gene Full Name | myocyte enhancer factor 2Ap |
| Gene Summary | The protein encoded by this gene is a DNA-binding transcription factor that activates many muscle-specific, growth factor-induced, and stress-induced genes. The encoded protein can act as a homodimer or as a heterodimer and is involved in several cellular processes, including muscle development, neuronal differentiation, cell growth control, and apoptosis. Defects in this gene could be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010] |
| Alternative Names | ADCAD1 antibody MADS box transcription enhancer factor 2 polypeptide A (myocyte enhancer factor 2A) antibody MEF2 antibody MEF2A antibody MEF2A_HUMAN antibody Myocyte enhancer factor 2A antibody Myocyte-specific enhancer factor 2A antibody RSRFC4 antibody RSRFC9 antibody Serum response factor like protein 1 antibody Serum response factor-like protein 1 antibody C5DELq14.3 antibody DEL5q14.3 antibody MADS box transcription enhancer factor 2 polypeptide C (myocyte enhancer factor 2C) antibody MADS box transcription enhancer factor 2 polypeptide C antibody MEF2C antibody MEF2C_HUMAN antibody Myocyte enhancer factor 2C antibody Myocyte specific enhancer factor 2C antibody Myocyte-specific enhancer factor 2C antibody OTTHUMP00000222409 antibody Similar to MADS box transcription enhancer factor 2 polypeptide C antibody |
| Molecular Weight(MW) | 55/51 kDa |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of MEF2A + MEF2C on THP-1 cell lysate with Rabbit anti-MEF2A + MEF2C antibody at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 46 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: 1/2,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-MEF2A + MEF2C antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Rabbit anti-MEF2A + MEF2C antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
IHC
Immunohistochemical analysis of paraffin-embedded rat brain tissue with Rabbit anti-MEF2A + MEF2C antibody at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
Immunocytochemistry analysis of THP-1 cells labeling MEF2A + MEF2C with Rabbit anti-MEF2A + MEF2C antibody at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-MEF2A + MEF2C antibody at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (HA1126) was used as the secondary antibody at 1/1,000 dilution.
FC
Flow cytometric analysis of Daudi cells labeling MEF2A + MEF2C. Cells were fixed and permeabilized. Then stained with the primary antibody (1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
IP
MEF2A + MEF2C was immunoprecipitated from 0.2 mg THP-1 cell lysate with Recombinant Rabbit Monoclonal Antibody at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using Recombinant Rabbit Monoclonal Antibody at 1/2,000 dilution. HRP Conjugated Anti-Rabbit IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: THP-1 cell lysate (input). Lane 2: Recombinant Rabbit Monoclonal Antibody IP in THP-1 cell lysate. Lane 3: Rabbit IgG instead of Recombinant Rabbit Monoclonal Antibody in THP-1 cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST. Exposure time: 3 minutes;| Application Notes | WB:1:2000 IHC:1:400-1:1000 ICC/IF:1:100 FC:1:500-1:1000 IP:1-2μg/sample |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
