IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using F4/80 antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody at dilution of 1:1500 and AF488-Conjugated Goat Anti-Rabbit IgG(H+L).IF-F
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse spleen tissue using F4/80 antibody at dilution of 1:200 and AF488-Conjugated Goat Anti-Rabbit IgG(H+L).ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using F4/80 antibody at dilution of 1:250 and AF488-Conjugated Goat Anti-Rabbit IgG(H+L).| Product Name | F4/80 Recombinant monoclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant mouse Adgre1 protein |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIF-FIF-PIHC |
| IHC:1:2000-1:3000 IF-P:1:750-1:3000 IF-F:1:50-1:500 ICC/IF:1:125-1:500 |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | Adgre1 |
|---|---|
| Gene Synonyms | Emr1 Ly71 F4/80 Gpf480 TM7LN3 DD7A5-7 EGF-TM7 |
| Gene Full Name | adhesion G protein-coupled receptor E1 |
| Gene Summary | Predicted to enable G protein-coupled receptor activity. Predicted to be involved in adenylate cyclase-activating G protein-coupled receptor signaling pathway. Located in external side of plasma membrane. Is expressed in several structures, including cardiovascular system; central nervous system; genitourinary system; hemolymphoid system; and intestine. Orthologous to human ADGRE1 (adhesion G protein-coupled receptor E1). [provided by Alliance of Genome Resources, Apr 2025] |
| Alternative Names | 2P18 Adgre1 Adhesion G protein-coupled receptor E1 EGF-like module receptor 1 EGF-like module-containing mucin-like hormone receptor-like 1 |
| Molecular Weight(MW) | 102 kDa |
| Cellular Localization | Cell membrane |
IHC
Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using F4/80 antibody at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF-P
Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using F4/80 antibody at dilution of 1:1500 and AF488-Conjugated Goat Anti-Rabbit IgG(H+L).
IF-F
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse spleen tissue using F4/80 antibody at dilution of 1:200 and AF488-Conjugated Goat Anti-Rabbit IgG(H+L).
ICC/IF
Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using F4/80 antibody at dilution of 1:250 and AF488-Conjugated Goat Anti-Rabbit IgG(H+L).| Application Notes | IHC:1:2000-1:3000 IF-P:1:750-1:3000 IF-F:1:50-1:500 ICC/IF:1:125-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
