WB
Western blot analysis of IL-13 Receptor alpha 1 on different lysates with Rabbit anti-IL-13 Receptor alpha 1 antibody at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Exposure time: 10 second; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IL-13 Receptor alpha 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.ICC/IF
ICC staining of IL-13 Receptor alpha 1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/100) for 1 hour at room temperature, washed with PBS. Alexa 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).| Product Name | IL-13 Receptor alpha 1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within Human IL13 Receptor alpha 1 aa 1-127 / 427. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:1000-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | IL13RA1 |
|---|---|
| Gene Synonyms | NR4 CT19 CD213A1 IL-13Ra |
| Gene Full Name | interleukin 13 receptor subunit alpha 1 |
| Gene Summary | The protein encoded by this gene is a subunit of the interleukin 13 receptor. This subunit forms a receptor complex with IL4 receptor alpha, a subunit shared by IL13 and IL4 receptors. This subunit serves as a primary IL13-binding subunit of the IL13 receptor, and may also be a component of IL4 receptors. This protein has been shown to bind tyrosine kinase TYK2, and thus may mediate the signaling processes that lead to the activation of JAK1, STAT3 and STAT6 induced by IL13 and IL4. [provided by RefSeq, Jul 2008] |
| Alternative Names | bB128O4.2.1 (interleukin 13 receptor alpha 1) antibody Cancer/testis antigen 19 antibody CD213a1 antibody CD213A1 Antigen antibody CT 19 antibody CT19 antibody I13R1_HUMAN antibody IL 13 receptor subunit alpha 1 antibody IL 13R alpha 1 antibody IL 13R subunit alpha 1 antibody IL 13Ra antibody IL 13RA1 antibody IL-13 receptor subunit alpha-1 antibody IL-13R subunit alpha-1 antibody IL-13R-alpha-1 antibody IL-13RA1 antibody IL13 receptor alpha 1 chain antibody IL13R antibody IL13RA antibody Il13ra1 antibody Interleukin 13 receptor alpha 1 antibody Interleukin 13 receptor alpha 1 chain antibody interleukin 13 receptor subunit alpha 1 antibody Interleukin-13 receptor subunit alpha-1 antibody NR 4 antibody NR4 antibody |
| Molecular Weight(MW) | 49 kDa |
| Cellular Localization | Membrane. |
WB
Western blot analysis of IL-13 Receptor alpha 1 on different lysates with Rabbit anti-IL-13 Receptor alpha 1 antibody at 1/1,000 dilution. Lysates/proteins at 40 µg/Lane. Exposure time: 10 second; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
IHC
Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-IL-13 Receptor alpha 1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ICC/IF
ICC staining of IL-13 Receptor alpha 1 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/100) for 1 hour at room temperature, washed with PBS. Alexa 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).| Application Notes | WB:1:1000-1:2000 IHC:1:50-1:200 ICC/IF:1:100-1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
