WB
Western Blot.
Positive WB detected in:Mouse endometrium tissue lysate, Rat Fallopian tube tissue lysate.
All lanes: DNALI1 antibody at 1:1000.
Secondary:
Goat polyclonal to rabbit IgG at 1/50000 dilution.
WB
Western Blot.
Positive WB detected in:PC-3 cell lysate.
All lanes: DNALI1 antibody at 1:1000.
Secondary:
Goat polyclonal to rabbit IgG at 1/50000 dilution.
IHC
IHC image of Rabbit anti-DNALI1 Polyclonal antibody diluted at 1:200 and staining in paraffin-embedded mouse testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control: uses 1% BSA instead of primary antibody.