WB
Western blot analysis of Apolipoprotein B on different lysates with Rabbit anti-Apolipoprotein B antibody at 1/8,000 dilution. Lysates/proteins at 40 µg/Lane. Exposure time: 3 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/8,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
Relative expression (RE) Western blot analysis of Apolipoprotein B on different lysates with Rabbit anti-Apolipoprotein B antibody at 1/10,000 dilution. Exposure time: Lane 1-2: 30 seconds; Lane 3-5: 2 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/10,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | Apolipoprotein B Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant protein within mouse Apolipoprotein B aa 371-830. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:8000-1:10000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | APOB |
|---|---|
| Gene Synonyms | FLDB FCHL2 LDLCQ4 apoB-48 apoB-100 |
| Gene Full Name | apolipoprotein B |
| Gene Summary | This gene product is the main apolipoprotein of chylomicrons and low density lipoproteins (LDL), and is the ligand for the LDL receptor. It occurs in plasma as two main isoforms, apoB-48 and apoB-100: the former is synthesized exclusively in the gut and the latter in the liver. The intestinal and the hepatic forms of apoB are encoded by a single gene from a single, very long mRNA. The two isoforms share a common N-terminal sequence. The shorter apoB-48 protein is produced after RNA editing of the apoB-100 transcript at residue 2180 (CAA->UAA), resulting in the creation of a stop codon, and early translation termination. Mutations in this gene or its regulatory region cause hypobetalipoproteinemia, normotriglyceridemic hypobetalipoproteinemia, and hypercholesterolemia due to ligand-defective apoB, diseases affecting plasma cholesterol and apoB levels. [provided by RefSeq, Dec 2019] |
| Alternative Names | Apo B 100 antibody Apo B antibody Apo B-100 antibody Apo B-48 antibody Apo B100 antibody Apo B48 antibody ApoB 100 antibody ApoB 48 antibody APOB antibody APOB_HUMAN antibody Apolipoprotein B (including Ag(x) antigen) antibody Apolipoprotein B 100 antibody Apolipoprotein B 48 antibody Apolipoprotein B antibody Apolipoprotein B-48 antibody Apolipoprotein B100 antibody Apolipoprotein B48 antibody FLDB antibody LDLCQ4 antibody |
| Molecular Weight(MW) | 516/250 kDa |
| Cellular Localization | Cytoplasm, Secreted, Lipid droplet. |
WB
Western blot analysis of Apolipoprotein B on different lysates with Rabbit anti-Apolipoprotein B antibody at 1/8,000 dilution. Lysates/proteins at 40 µg/Lane. Exposure time: 3 seconds; 3-8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/8,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Relative expression (RE) Western blot analysis of Apolipoprotein B on different lysates with Rabbit anti-Apolipoprotein B antibody at 1/10,000 dilution. Exposure time: Lane 1-2: 30 seconds; Lane 3-5: 2 minutes 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/10,000 dilution was used in primary antibody dilution at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:8000-1:10000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
