WB
Western blot analysis of ALDH16A1 on different lysates with Rabbit anti-ALDH16A1 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: Mouse spleen tissue lysate (40 µg/Lane) Lane 5: Rat spleen tissue lysate (40 µg/Lane) Exposure time: 1 minute 7 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.WB
Western blot analysis of ALDH16A1 on different lysates with Rabbit anti-ALDH16A1 antibody at 1/1,000 dilution. Lane 1: A549-WT cell lysate, Lane 2: A549-KD ALDH16A1 cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Product Name | ALDH16A1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within human ALDH16A1 aa 550-600/802. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500-1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | ALDH16A1 |
|---|---|
| Gene Full Name | aldehyde dehydrogenase 16 family member A1 |
| Gene Summary | This gene encodes a member of the aldehyde dehydrogenase superfamily. The family members act on aldehyde substrates and use nicotinamide adenine dinucleotide phosphate (NADP) as a cofactor. This gene is conserved in chimpanzee, dog, cow, mouse, rat, and zebrafish. The protein encoded by this gene interacts with maspardin, a protein that when truncated is responsible for Mast syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2010] |
| Alternative Names |
Aldehyde dehydrogenase 16 family member A1 antibody Aldehyde dehydrogenase family 16 member A1 antibody ALDH16A1 aldehyde dehydrogenase 16 family member A1 antibody Aldh16a1 antibody MGC10204 antibody |
| Molecular Weight(MW) | 85 kDaObserved band size: 75 kDa |
| Cellular Localization | Membrane. |
WB
Western blot analysis of ALDH16A1 on different lysates with Rabbit anti-ALDH16A1 antibody at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A549 cell lysate (20 µg/Lane) Lane 3: HepG2 cell lysate (20 µg/Lane) Lane 4: Mouse spleen tissue lysate (40 µg/Lane) Lane 5: Rat spleen tissue lysate (40 µg/Lane) Exposure time: 1 minute 7 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
WB
Western blot analysis of ALDH16A1 on different lysates with Rabbit anti-ALDH16A1 antibody at 1/1,000 dilution. Lane 1: A549-WT cell lysate, Lane 2: A549-KD ALDH16A1 cell lysate, Lysates/proteins at 10 µg/Lane. Exposure time: 2 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:500-1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
