WB
Western blot analysis of Claudin 2/CLDN2 using anti-Claudin 2/CLDN2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Claudin 2/CLDN2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.ICC/IF
ICC/IF analysis of Claudin 2/CLDN2 using anti-Claudin 2/CLDN2 antibody. Claudin 2/CLDN2 was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with rabbit anti-Claudin 2/CLDN2 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI.FC
Flow Cytometry analysis of A549 cells using anti-Claudin 2/CLDN2 antibody. Overlay histogram showing A549 cells stained with Rabbit polyclonal antibody to Claudin 2/CLDN2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Claudin 2/CLDN2 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Product Name | Rabbit polyclonal antibody to Claudin 2/CLDN2 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human CLDN2 recombinant protein (Position: Q63-V230). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FCICC/IFWB |
| WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CLDN2 |
|---|---|
| Gene Synonyms | OAZON claudin-2 |
| Gene Full Name | claudin 2 |
| Gene Summary | This gene product belongs to the claudin protein family whose members have been identified as major integral membrane proteins localized exclusively at tight junctions. Claudins are expressed in an organ-specific manner and regulate tissue-specific physiologic properties of tight junctions. This protein is expressed in the intestine. Alternatively spliced transcript variants with different 5' untranslated region have been found for this gene.[provided by RefSeq, Jan 2010] |
| Alternative Names | claudin 2 CLDN2 SP82 UNQ705/PRO1356 |
| Molecular Weight(MW) | 25 kDa |
| Cellular Localization | Cell junction,Cell membrane,Membrane,Tight junction. |
WB
Western blot analysis of Claudin 2/CLDN2 using anti-Claudin 2/CLDN2 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Claudin 2/CLDN2 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
ICC/IF
ICC/IF analysis of Claudin 2/CLDN2 using anti-Claudin 2/CLDN2 antibody. Claudin 2/CLDN2 was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with rabbit anti-Claudin 2/CLDN2 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI.
FC
Flow Cytometry analysis of A549 cells using anti-Claudin 2/CLDN2 antibody. Overlay histogram showing A549 cells stained with Rabbit polyclonal antibody to Claudin 2/CLDN2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-Claudin 2/CLDN2 Antibody at 1:100 dilution for 30 min at 20°C. Fluoro488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.| Application Notes | WB:1:500-1:2000 ICC/IF:1:50-1:400 FC:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
