WB
Western blot analysis of ACOX1 using anti-ACOX1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACOX1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of ACOX1 using anti-ACOX1 antibody. ACOX1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-ACOX1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of ACOX1 using anti-ACOX1 antibody. ACOX1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-ACOX1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Product Name | Rabbit polyclonal antibody to ACOX1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human ACOX1 recombinant protein (Position: A605-E648). Human ACOX1 shares 93.2% and 90.9% amino acid (aa) sequence identity with mouse and rat ACOX1, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | ACOX1 |
|---|---|
| Gene Synonyms | AOX ACOX SCOX MITCH PALMCOX |
| Gene Full Name | acyl-CoA oxidase 1 |
| Gene Summary | The protein encoded by this gene is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-CoAs to 2-trans-enoyl-CoAs. It donates electrons directly to molecular oxygen, thereby producing hydrogen peroxide. Defects in this gene result in pseudoneonatal adrenoleukodystrophy, a disease that is characterized by accumulation of very long chain fatty acids. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008] |
| Alternative Names | Peroxisomal acyl-CoA oxidase 1 A chain Palmitoyl-CoA oxidase EC:1.3.3.6 AOX ACOX 1 |
| Molecular Weight(MW) | 74 kDa(Observed band size:74, 22 kDa) |
| Cellular Localization | Peroxisome. |
WB
Western blot analysis of ACOX1 using anti-ACOX1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ACOX1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of ACOX1 using anti-ACOX1 antibody. ACOX1 was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-ACOX1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of ACOX1 using anti-ACOX1 antibody. ACOX1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-ACOX1 Antibody at a dilution of 1:100. Fluoro488 Conjugated Goat Anti-Rabbit IgG (Green) was used as secondary antibody. The section was counterstained with DAPI (Blue).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
