WB
Western blot analysis of lysates from HeLa cells treated with Ca+ 40nM 30', using NFAT4 (Phospho-Ser165) Antibody. The lane on the right is blocked with the phospho peptide.IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4℃ overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).ICC/IF
Immunofluorescence analysis of HeLa cells, using NFAT4 (Phospho-Ser165) Antibody. The picture on the right is blocked with the phospho peptide.| Product Name | NFATc3 (Phospho Ser165) Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NFAT4 around the phosphorylation site of Ser165. AA range:131-180. |
| Modification | p-Ser165 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NFATC3 |
|---|---|
| Gene Synonyms | NFAT4 NFATX NF-AT4c n339260 |
| Gene Full Name | nuclear factor of activated T cells 3 |
| Gene Summary | The product of this gene is a member of the nuclear factors of activated T cells DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor (TCR) stimulation and an inducible nuclear component. Other members of this family participate to form this complex also. The product of this gene plays a role in the regulation of gene expression in T cells and immature thymocytes. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Nov 2010] |
| Alternative Names | NFATC3 NFAT4 Nuclear factor of activated T-cells cytoplasmic 3 NF-ATc3 NFATc3 NFATx T-cell transcription factor NFAT4 NF-AT4 |
| Molecular Weight(MW) | 115 kDa |
| Cellular Localization | Cytoplasm . Nucleus . Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. |
WB
Western blot analysis of lysates from HeLa cells treated with Ca+ 40nM 30', using NFAT4 (Phospho-Ser165) Antibody. The lane on the right is blocked with the phospho peptide.
IHC
Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4℃ overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
ICC/IF
Immunofluorescence analysis of HeLa cells, using NFAT4 (Phospho-Ser165) Antibody. The picture on the right is blocked with the phospho peptide.| Application Notes | WB:1:500-1:2000 IHC:1:100-1:300 ICC/IF:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide. |
