WB
Western blot analysis of Mouse brain, using BNP antibody at 1:1000 dilution.IHC
Immunohistochemistry of paraffin-embedded mouse heart using BNP pAb at dilution of 1:200 (40x lens).ICC/IF
Immunofluorescence analysis of mouse heart cells using BNP pAb at dilution of 1:150 (40x lens).ICC/IF
Immunofluorescence analysis of rat heart cells using BNP pAb at dilu tion of 1:150 (40x lens).| Product Name | BNP polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-134 of human BNP (NP_002512.1). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NPPB |
|---|---|
| Gene Synonyms | BNP Iso-ANP |
| Gene Full Name | natriuretic peptide B |
| Gene Summary | This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The presence of myocardial injury is a significant predictor of mortality in hospitalized coronavirus disease 2019 (COVID-19) patients, and there is evidence of increased levels of natriuretic peptide B in hospitalized non-survivor COVID-19 patients. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Aug 2020] |
| Alternative Names | NPPB BNP |
| Molecular Weight(MW) | 15 KDa |
| Cellular Localization | Secreted. |
WB
Western blot analysis of Mouse brain, using BNP antibody at 1:1000 dilution.
IHC
Immunohistochemistry of paraffin-embedded mouse heart using BNP pAb at dilution of 1:200 (40x lens).
ICC/IF
Immunofluorescence analysis of mouse heart cells using BNP pAb at dilution of 1:150 (40x lens).
ICC/IF
Immunofluorescence analysis of rat heart cells using BNP pAb at dilu tion of 1:150 (40x lens).| Application Notes | WB:1:500-1:2000 IHC:1:50-1:200 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. |
| Storage Buffer | 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2. |
