WB
Western blot analysis of OGG1 using anti-OGG1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OGG1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of OGG1 using anti-OGG1 antibody. OGG1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-OGG1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of OGG1 using anti-OGG1 antibody. OGG1 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-OGG1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of OGG1 using anti-OGG1 antibody and anti-Beta Tubulin antibody. OGG1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-OGG1 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.WB
IP analysis of OGG1 using anti-OGG1 antibody in 293T whole cell lysate. Western blot analysis of OGG1 using anti- OGG1 antibody. Lane 1: 293T whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- OGG1 antibody in 293T whole cell lysate, Lane 3: anti- OGG1 antibody (2μg) + 293T whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- OGG1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Product Name | Rabbit polyclonal antibody to OGG1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the N-terminus of human Ogg1, which shares 89.7% and 87.2% amino acid (aa) sequence identity with mouse and rat Ogg1, respectively. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IP:1:250-1:300 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | OGG1 |
|---|---|
| Gene Synonyms | HMMH MUTM OGH1 HOGG1 |
| Gene Full Name | 8-oxoguanine DNA glycosylase |
| Gene Summary | This gene encodes the enzyme responsible for the excision of 8-oxoguanine, a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen. The action of this enzyme includes lyase activity for chain cleavage. Alternative splicing of the C-terminal region of this gene classifies splice variants into two major groups, type 1 and type 2, depending on the last exon of the sequence. Type 1 alternative splice variants end with exon 7 and type 2 end with exon 8. All variants share the N-terminal region in common, which contains a mitochondrial targeting signal that is essential for mitochondrial localization. Many alternative splice variants for this gene have been described, but the full-length nature for every variant has not been determined. [provided by RefSeq, Aug 2008] |
| Alternative Names | 8 oxoguanine DNA glycosylase AP lyase HMMH HOGG1 MUTM OGG1 OGH1 |
| Molecular Weight(MW) | 39 kDa |
| Cellular Localization | Mitochondrion,Nucleus |
WB
Western blot analysis of OGG1 using anti-OGG1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-OGG1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of OGG1 using anti-OGG1 antibody. OGG1 was detected in a paraffin-embedded section of human breast cancer tissue. The tissue section was incubated with rabbit anti-OGG1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of OGG1 using anti-OGG1 antibody. OGG1 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-OGG1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of OGG1 using anti-OGG1 antibody and anti-Beta Tubulin antibody. OGG1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-OGG1 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.
WB
IP analysis of OGG1 using anti-OGG1 antibody in 293T whole cell lysate. Western blot analysis of OGG1 using anti- OGG1 antibody. Lane 1: 293T whole cell lysates(30ug), Lane 2: Rabbit control IgG instead of anti- OGG1 antibody in 293T whole cell lysate, Lane 3: anti- OGG1 antibody (2μg) + 293T whole cell lysate (500μg). After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti- OGG1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 IP:1:250-1:300 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. |
| Storage Buffer | 500ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
