WB
Cell treatment (CT) Western blot analysis of Phospho-Aurora A (T288) on different lysates with Rabbit anti-Phospho-Aurora A (T288) antibody at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 20 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: 1/5,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.| Product Name | Phospho-Aurora A (T288) Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Thr288 of human Aurora A. |
| Modification | p-T288 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:5000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | AURKA |
|---|---|
| Gene Synonyms | AIK ARK1 AURA BTAK STK6 STK7 STK15 PPP1R47 |
| Gene Full Name | aurora kinase A |
| Gene Summary | The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| Alternative Names | AIK antibody ARK-1 antibody ARK1 antibody AURA antibody Aurka antibody Aurora 2 antibody Aurora A antibody Aurora kinase A antibody Aurora-related kinase 1 antibody Aurora/IPL1 like kinase antibody Aurora/IPL1-related kinase 1 antibody AURORA2 antibody Breast tumor-amplified kinase antibody BTAK antibody hARK1 antibody IAK antibody IPL1 related kinase antibody MGC34538 antibody OTTHUMP00000031340 antibody OTTHUMP00000031341 antibody OTTHUMP00000031342 antibody OTTHUMP00000031343 antibody OTTHUMP00000031344 antibody OTTHUMP00000031345 antibody OTTHUMP00000166071 antibody OTTHUMP00000166072 antibody PPP1R47 antibody Protein phosphatase 1 regulatory subunit 47 antibody Serine/threonine kinase 15 antibody Serine/threonine kinase 6 antibody Serine/threonine-protein kinase 15 antibody Serine/threonine-protein kinase 6 antibody Serine/threonine-protein kinase aurora-A antibody STK15 antibody STK6 antibody STK6_HUMAN antibody |
| Molecular Weight(MW) | 46 kDa(Observed band size:48 kDa) |
| Cellular Localization | Cytoplasm, Cytoskeleton, Nucleus, Cell projection, Microtubule. |
WB
Cell treatment (CT) Western blot analysis of Phospho-Aurora A (T288) on different lysates with Rabbit anti-Phospho-Aurora A (T288) antibody at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 20 seconds; Blocking: 5% NFDM/TBST, 1 hour at room temperature. Primary antibody: 1/5,000 in primary antibody dilution buffer, overnight at 4 °C. Secondary antibody: Goat anti-Rabbit IgG-HRP, 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature.| Application Notes | WB:1:5000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*PBS (pH7.4), 0.1% BSA, 40% Glycerol, 0.2% Proclean 950. |
