WB
Western blot analysis of C5AR1 using anti-C5AR1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-C5AR1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of C5AR1 using anti-C5AR1 antibody. C5AR1 was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-C5AR1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Product Name | Rabbit polyclonal antibody to C5AR1 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | E.coli-derived human C5AR1 recombinant protein (Position: F254-V350). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | C5AR1 |
|---|---|
| Gene Synonyms | C5A C5AR C5R1 CD88 |
| Gene Full Name | complement C5a receptor 1 |
| Gene Summary | Enables G protein-coupled receptor activity and complement component C5a receptor activity. Involved in several processes, including complement component C5a signaling pathway; mRNA transcription by RNA polymerase II; and positive regulation of ERK1 and ERK2 cascade. Located in apical part of cell and basolateral plasma membrane. Biomarker of Alzheimer's disease; asthma; chronic obstructive pulmonary disease; rhinitis; and severe acute respiratory syndrome. [provided by Alliance of Genome Resources, Jul 2025] |
| Alternative Names | C5A C5a R C5aR C5AR1 C5R1 CD88 |
| Molecular Weight(MW) | 39 kDa(Observed band size:45 kDa) |
| Cellular Localization | Cell membrane,Cytoplasmic vesicle,Membrane. |
WB
Western blot analysis of C5AR1 using anti-C5AR1 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-C5AR1 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of C5AR1 using anti-C5AR1 antibody. C5AR1 was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-C5AR1 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt，-20℃ as supplied. |
| Storage Buffer | 500 ug/ml antibody with PBS，0.02% NaN3, 1 mg BSA and 50% glycerol. |
