WB
Western blot analysis of lysates from NIH3T3 cells, using Phospho-TrkA-Y490 Rabbit pAb. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA.WB
Western blot analysis of lysates from PC-12 cells using Phospho-TrkA-Y490 Rabbit pAb at 1:1800 dilution. PC-12 cells were treated by MNGF (200 ng/ml) at 37℃ for 10 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.| Product Name | Phospho-TrkA-Y490 Rabbit pAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide. This information is considered to be commercially sensitive. |
| Modification | p-Y490 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:500 - 1:2000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NTRK1 |
|---|---|
| Gene Synonyms | MTC TRK TRK1 TRKA Trk-A p140-TrkA |
| Gene Full Name | neurotrophic receptor tyrosine kinase 1 |
| Gene Summary | This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, cognitive disability and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. [provided by RefSeq, Jul 2008] |
| Alternative Names | MTC TRK TRK1 TRKA Trk-A p140-TrkA Phospho-TrkA-Y490 |
| Molecular Weight(MW) | 87 kDa(Observed band size:150 kDa/140 kDa) |
| Cellular Localization | Cell membrane, Early endosome membrane, Late endosome membrane, Single-pass type I membrane protein. |
WB
Western blot analysis of lysates from NIH3T3 cells, using Phospho-TrkA-Y490 Rabbit pAb. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% BSA.
WB
Western blot analysis of lysates from PC-12 cells using Phospho-TrkA-Y490 Rabbit pAb at 1:1800 dilution. PC-12 cells were treated by MNGF (200 ng/ml) at 37℃ for 10 minutes after serum-starvation overnight. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.| Application Notes | WB:1:500 - 1:2000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS containing 50% glycerol, preserved with proclin300 or sodium azide (as specified on the Certificate of Analysis), pH 7.3. |
