WB
WB analysis using EIF4G3 Polyclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with EIF4G3 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.WB
WB analysis of HEK-293 using EIF4G3 Polyclonal antibody. HEK-293 cells were subjected to SDS PAGE followed by western blot with EIF4G3 Antibody at dilution of 1:600 incubated at 4 degree celsius over night.IHC
IHC staining of human gliomas using EIF4G3 Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded human gliomas using EIF4G3 antibody at dilution of 1:10 (under 10x lens).ICC/IF
IF Staining of HeLa using EIF4G3 Polyclonal antibody. Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF4G3 antibody at dilution of 1:400 and 488-Conjugated Goat Anti-Rabbit IgG(H+L).IP
IP experiment of HeLa using EIF4G3 Polyclonal antibody. IP result of anti-EIF4G3 (4ug; 1:500) with HeLa cells lysate 4000ug.| Product Name | EIF4G3 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human EIF4G3 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:1000-1:6000 IHC:1:10-1:100 ICC/IF:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | EIF4G3 |
|---|---|
| Gene Synonyms | eIF4G 3 eIF4GII eIF-4G 3 |
| Gene Full Name | eukaryotic translation initiation factor 4 gamma 3 |
| Gene Summary | The protein encoded by this gene is thought to be part of the eIF4F protein complex, which is involved in mRNA cap recognition and transport of mRNAs to the ribosome. Interestingly, a microRNA (miR-520c-3p) has been found that negatively regulates synthesis of the encoded protein, and this leads to a global decrease in protein translation and cell proliferation. Therefore, this protein is a key component of the anti-tumor activity of miR-520c-3p. [provided by RefSeq, May 2016] |
| Alternative Names | eIF4GII eIF-4-gamma II eIF-4-gamma 3 eIF-4G 3 eIF4G 3 |
| Molecular Weight(MW) | 177 kDa(Observed band size:70 kDa, 250 kDa) |
WB
WB analysis using EIF4G3 Polyclonal antibody. Various lysates were subjected to SDS PAGE followed by western blot with EIF4G3 antibody at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB
WB analysis of HEK-293 using EIF4G3 Polyclonal antibody. HEK-293 cells were subjected to SDS PAGE followed by western blot with EIF4G3 Antibody at dilution of 1:600 incubated at 4 degree celsius over night.
IHC
IHC staining of human gliomas using EIF4G3 Polyclonal antibody. Immunohistochemical analysis of paraffin-embedded human gliomas using EIF4G3 antibody at dilution of 1:10 (under 10x lens).
ICC/IF
IF Staining of HeLa using EIF4G3 Polyclonal antibody. Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using EIF4G3 antibody at dilution of 1:400 and 488-Conjugated Goat Anti-Rabbit IgG(H+L).
IP
IP experiment of HeLa using EIF4G3 Polyclonal antibody. IP result of anti-EIF4G3 (4ug; 1:500) with HeLa cells lysate 4000ug.| Application Notes | WB:1:1000-1:6000 IHC:1:10-1:100 ICC/IF:1:200-1:800 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
