IHC
IHC image of Rabbit anti-UNC5B Polyclonal antibody diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.ICC/IF
Immunofluorescence staining of HepG2 cells with Rabbit anti-UNC5B Polyclonal antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Product Name | Rabbit anti-UNC5B Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant Human Netrin receptor UNC5B protein (404-517AA). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC |
| IHC:1:200-1:500 ICC/IF:1:50-1:200 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein G |
| Gene Symbol | UNC5B |
|---|---|
| Gene Synonyms | UNC5H2 p53RDL1 |
| Gene Full Name | unc-5 netrin receptor B |
| Gene Summary | This gene encodes a member of the netrin family of receptors. This particular protein mediates the repulsive effect of netrin-1 and is a vascular netrin receptor. This encoded protein is also in a group of proteins called dependence receptors (DpRs) which are involved in pro- and anti-apoptotic processes. Many DpRs are involved in embryogenesis and in cancer progression. Two alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2011] |
| Alternative Names | 6330415E02Rik antibody A630020F16 antibody D10Bwg0792e antibody Netrin receptor UNC5B antibody Netrin receptor UNC5B Precursor antibody p53 regulated receptor for death and life antibody p53 regulated receptor for death and life protein 1 antibody p53-regulated receptor for death and life protein 1 antibody p53RDL1 antibody Protein unc-5 homolog 2 antibody Protein unc-5 homolog B antibody Protein XUNC 5 antibody Transmembrane receptor Unc5H2 antibody Unc 5 homolog 2 antibody Unc 5 homolog B antibody Unc 5 netrin receptor B antibody UNC 5B antibody Unc5 (C.elegans homolog) b antibody Unc5b antibody UNC5B_HUMAN antibody UNC5H2 antibody |
| Cellular Localization | Cell membrane,Membrane. |
IHC
IHC image of Rabbit anti-UNC5B Polyclonal antibody diluted at 1:300 and staining in paraffin-embedded human lung tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
ICC/IF
Immunofluorescence staining of HepG2 cells with Rabbit anti-UNC5B Polyclonal antibody at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).| Application Notes | IHC:1:200-1:500 ICC/IF:1:50-1:200 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. |
| Storage Buffer | Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH 7.4. |
