WB
HEK-293 cells were subjected to SDS PAGE followed by western blot with COA6 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.WB
mouse liver tissue were subjected to SDS PAGE followed by western blot with COA6 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using COA6 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C1orf31 antibody at dilution of 1:200 and 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).IP
IP result of anti-COA6 (IP:4ug; Detection:1:500) with HEK-293 cells lysate 1760ug.| Product Name | COA6 Polyclonal antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Recombinant human C1orf31 protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| WB:1:500-1:2000 IHC:1:500-1:2000 ICC/IF:1:200 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | COA6 |
|---|---|
| Gene Synonyms | C1orf31 CEMCOX4 MC4DN13 |
| Gene Full Name | cytochrome c oxidase assembly factor 6 |
| Gene Summary | This gene encodes a member of the cytochrome c oxidase subunit 6B family. The encoded protein associates with cytochrome c oxidase may act has an cytochrome c oxidase mitochondrial respiratory complex VI assembly factor. Mutations in this gene may be associated with fatal infantile cardiomyopathy. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014] |
| Alternative Names | C1orf31 |
| Molecular Weight(MW) | 10-18 kDa |
| Cellular Localization | Mitochondrion |
WB
HEK-293 cells were subjected to SDS PAGE followed by western blot with COA6 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB
mouse liver tissue were subjected to SDS PAGE followed by western blot with COA6 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using COA6 antibody at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
ICC/IF
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using C1orf31 antibody at dilution of 1:200 and 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IP
IP result of anti-COA6 (IP:4ug; Detection:1:500) with HEK-293 cells lysate 1760ug.| Application Notes | WB:1:500-1:2000 IHC:1:500-1:2000 ICC/IF:1:200 IP:0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
