WB
Western blot analysis of MBNL1 on different lysates with Rabbit anti-MBNL1 antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes 33 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.| Product Name | MBNL1 Recombinant Rabbit Monoclonal Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | Synthetic peptide within Human MBNL1 aa 339-388 / 388. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB:1:1000 |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | MBNL1 |
|---|---|
| Gene Synonyms | EXP MBNL |
| Gene Full Name | muscleblind like splicing regulator 1 |
| Gene Summary | This gene encodes a member of the muscleblind protein family which was initially described in Drosophila melanogaster. The encoded protein is a C3H-type zinc finger protein that modulates alternative splicing of pre-mRNAs. Muscleblind proteins bind specifically to expanded dsCUG RNA but not to normal size CUG repeats and may thereby play a role in the pathophysiology of myotonic dystrophy. Mice lacking this gene exhibited muscle abnormalities and cataracts. Several alternatively spliced transcript variants have been described but the full-length natures of only some have been determined. The different isoforms are thought to have different binding specificities and/or splicing activities. [provided by RefSeq, Sep 2015] |
| Alternative Names | EXP antibody EXP35 antibody EXP40 antibody EXP42 antibody KIAA0428 antibody MBNL antibody MBNL protein antibody MBNL1 antibody MBNL1_HUMAN antibody Muscleblind 41kD isoform antibody Muscleblind like antibody Muscleblind like protein 1 antibody Muscleblind like splicing regulator 1 antibody Muscleblind-like protein 1 antibody Triplet expansion RNA binding protein antibody Triplet-expansion RNA-binding protein antibody |
| Molecular Weight(MW) | 42 kDa(Observed band size:37/42 kDa) |
| Cellular Localization | Nucleus. Cytoplasm. |
WB
Western blot analysis of MBNL1 on different lysates with Rabbit anti-MBNL1 antibody at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 2 minutes 33 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.| Application Notes | WB:1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term. |
| Storage Buffer | 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide. |
