WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PLOD2 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.IHC
Human small intestine was stained with anti-PLOD2 Rabbit antibody.| Product Name | PLOD2 Rabbit mAb |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | The specific immunogen used to produce this antibody is proprietary information. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG,K |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB:1:2000-1:10000 IHC:1:200-1:1000 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PLOD2 |
|---|---|
| Gene Synonyms | LH2 TLH BRKS2 |
| Gene Full Name | procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 |
| Gene Summary | The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| Molecular Weight(MW) | 85 kDa(Observed band size: 95 kDa) |
| Cellular Localization | Rough endoplasmic reticulum membrane; Peripheral membrane protein; Lumenal side. |
WB
Various whole cell lysates were separated by 4-20% SDS-PAGE, and the membrane was blotted with anti-PLOD2 antibody. The HRP-conjugated Goat anti-Rabbit IgG (H + L) antibody was used to detect the antibody.
IHC
Human small intestine was stained with anti-PLOD2 Rabbit antibody.| Application Notes | WB:1:2000-1:10000 IHC:1:200-1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | -15°C to -25°C/1 year (Do not lower than -25°C). |
| Storage Buffer | PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA. |
