WB
Western blot analysis of lysates from HepG2 cells, using GLUT3/SLC2A3 Rabbit pAb at 1:500
dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
IHC
Immunohistochemistry analysis of paraffin
embedded Rat brain using GLUT3/SLC2A3
Rabbit pAb at dilution of 1:50 (40x
lens). High pressure antigen retrieval
performed with 0.01M Citrate buffer (pH 6.0)
prior to IHC staining.