WB
Western blot analysis of RBM10 using anti-RBM10 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RBM10 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.IHC
IHC analysis of RBM10 using anti-RBM10 antibody. RBM10 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-RBM10 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.IHC
IHC analysis of RBM10 using anti-RBM10 antibody. RBM10 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-RBM10 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.ICC/IF
ICC/IF analysis of RBM10 using anti-RBM10 antibody and anti-Alpha Tubulin antibody. RBM10 was detected in an immunocytochemical section of SIHA cells. The section was incubated with rabbit anti-RBM10 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.| Product Name | Rabbit polyclonal antibody to RBM10 |
|---|---|
| Antibody Type | Primary Antibodies |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human RBM10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCWB |
| WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Symbol | RBM10 |
|---|---|
| Gene Synonyms | S1-1 TARPS GPATC9 ZRANB5 GPATCH9 DXS8237E MINAS-60 |
| Gene Full Name | RNA binding motif protein 10 |
| Gene Summary | This gene encodes a nuclear protein that belongs to a family proteins that contain an RNA-binding motif. The encoded protein associates with hnRNP proteins and may be involved in regulating alternative splicing. Defects in this gene are the cause of the X-linked recessive disorder, TARP syndrome. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Mar 2011] |
| Alternative Names | DXS8237E G patch domain-containing protein 9 GPATC9 GPATCH9 KIAA0122 |
| Molecular Weight(MW) | 104 kDa(Observed band size:100-120 kDa) |
| Cellular Localization | Nucleus. |
WB
Western blot analysis of RBM10 using anti-RBM10 antibody. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RBM10 antigen affinity purified polyclonal antibody at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using ECL Plus Western Blotting Substrate.
IHC
IHC analysis of RBM10 using anti-RBM10 antibody. RBM10 was detected in a paraffin-embedded section of human lung cancer tissue. The tissue section was incubated with rabbit anti-RBM10 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
IHC
IHC analysis of RBM10 using anti-RBM10 antibody. RBM10 was detected in a paraffin-embedded section of rat brain tissue. The tissue section was incubated with rabbit anti-RBM10 Antibody at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.
ICC/IF
ICC/IF analysis of RBM10 using anti-RBM10 antibody and anti-Alpha Tubulin antibody. RBM10 was detected in an immunocytochemical section of SIHA cells. The section was incubated with rabbit anti-RBM10 Antibody at a dilution of 1:100. Fluoro488-conjugated Anti-rabbit IgG Secondary Antibody (green) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red) were used as secondary antibody.| Application Notes | WB:1:500-1:2000 IHC:1:50-1:400 ICC/IF:1:50-1:400 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | 12 months from date of receipt, -20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing. |
| Storage Buffer | 500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol. |
